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II类洞充填修复不同大块充填技术的微渗漏分析:µ-CT、扫描电子显微镜及能谱分析评估

Microleakage Analysis of Different Bulk-Filling Techniques for Class II Restorations: µ-CT, SEM and EDS Evaluations.

作者信息

Tosco Vincenzo, Vitiello Flavia, Furlani Michele, Gatto Maria Laura, Monterubbianesi Riccardo, Giuliani Alessandra, Orsini Giovanna, Putignano Angelo

机构信息

Department of Clinical Sciences and Stomatology (DISCO), Polytechnic University of Marche, 60126 Ancona, Italy.

Department of Materials, Environmental Sciences and Urban Planning (SIMAU), Polytechnic University of Marche, 60131 Ancona, Italy.

出版信息

Materials (Basel). 2020 Dec 23;14(1):31. doi: 10.3390/ma14010031.

Abstract

This study aimed to compare two different bulk-filling techniques, evaluating the internal and external adaptation of class II resin-composite restorations, by analysing the gap formation using microcomputed tomography (µ-CT) and scanning electronic microscopy (SEM) coupled with energy-dispersive X-ray spectroscopy (EDS). Two standardized mesio/disto-occlusal (MO/DO) cavities were prepared in eight extracted human third molars that were divided, according to the filling technique used, in the following two groups (n = 4): BG (Bulk&Go group) and BT (Bulk Traditional group). After universal bonding application, followed by the light curing, all teeth were restored using a bulk-fill composite. Specimens were scanned with µ-CT to evaluate 3D interfacial gaps. Acquired µ-CT data were analysed to quantify the gap formation. Complementary information to the µ-CT analysis were obtained by SEM. Thereafter, the chemical composition of tooth-restoration interface was analysed using EDS. The µ-CT analysis revealed gaps formation at the tooth-restoration interface for both the BG and BT groups, while within the restoration, only in the BT group there was evidence of microleakage formation. The scanning electron micrographs of both groups showed that the external marginal integrity of the restoration was preserved, while EDS showed the three different structures (tooth surface, adhesive layer and resin composite) of the tooth-restoration interface, highlighting the absence of gap formation. In both BG and BT, the two filling techniques did not show significant differences regarding the internal and external marginal adaptation of the restoration. To achieve a successful restoration, the clinician could be advised to restore a class II cavity using a single increment bulk-filling technique (BG), thus treating it as a class I cavity.

摘要

本研究旨在比较两种不同的大块充填技术,通过使用微观计算机断层扫描(µ-CT)和扫描电子显微镜(SEM)结合能量色散X射线光谱(EDS)分析间隙形成情况,评估II类树脂复合材料修复体的内部和外部适应性。在八颗拔除的人类第三磨牙上制备两个标准化的近中/远中咬合(MO/DO)洞型,根据使用的充填技术将其分为以下两组(n = 4):BG组(Bulk&Go组)和BT组(传统大块充填组)。在进行通用粘结后,进行光固化,所有牙齿均使用大块充填复合材料进行修复。用µ-CT扫描样本以评估三维界面间隙。对获取的µ-CT数据进行分析以量化间隙形成情况。通过SEM获得与µ-CT分析互补的信息。此后,使用EDS分析牙齿-修复体界面的化学成分。µ-CT分析显示BG组和BT组在牙齿-修复体界面均有间隙形成,而在修复体内,仅BT组有微渗漏形成的证据。两组的扫描电子显微镜图像均显示修复体的外部边缘完整性得以保留,而EDS显示了牙齿-修复体界面的三种不同结构(牙齿表面、粘结层和树脂复合材料),突出显示无间隙形成。在BG组和BT组中,两种充填技术在修复体的内部和外部边缘适应性方面均未显示出显著差异。为实现成功修复,建议临床医生使用单次增量大块充填技术(BG)修复II类洞型,从而将其视为I类洞型进行处理。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/089e/7793523/c639c45c84bb/materials-14-00031-g001.jpg

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