杀生聚合物：聚（天冬氨酸-琥珀酰亚胺）双季铵盐的合成、表征及抗菌活性

Biocidal Polymers: Synthesis, Characterization and Antimicrobial Activity of Bis-Quaternary Onium Salts of Poly(aspartate--succinimide).

作者信息

El-Newehy Mohamed H, A Meera Moydeen, Aldalbahi Ali K, Thamer Badr M, Mahmoud Yehia A-G, El-Hamshary Hany

机构信息

Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt.

出版信息

Polymers (Basel). 2020 Dec 23;13(1):23. doi: 10.3390/polym13010023.

DOI:10.3390/polym13010023

PMID:33374723

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7793505/

Abstract

Microbial multidrug resistance presents a real problem to human health. Therefore, water-soluble polymers based on poly(aspartate--succinimide) were synthesized via reaction of poly(aspartate--succinimide) with bis-quaternary ammonium or quaternary salts. The resultant copolymers were characterized by various techniques such as FTIR, TGA, HNMR, CNMR and elemental microanalysis. Antimicrobial activities of the new onium salts were investigated against , and the fungi; , and by agar diffusion method. Antimicrobial activity was studied in terms of inhibition zone diameters, in addition to the estimation of minimal inhibitory concentration (MIC) of the prepared compounds. and were the most affected microorganisms among the tested microorganisms with an inhibition zone of 19-21 (mm) in case of biocides, (V) and (VII). The obtained results showed that the quaternary onium salts have higher activity compared to the aspartate copolymer with MIC concentrations of 25 mg/mL for (VII) and (V) and 50 mg/mL for (VI) and (IV).

摘要

微生物的多重耐药性给人类健康带来了切实的问题。因此，通过聚（天冬氨酸-琥珀酰亚胺）与双季铵盐或季盐反应合成了基于聚（天冬氨酸-琥珀酰亚胺）的水溶性聚合物。通过傅里叶变换红外光谱（FTIR）、热重分析（TGA）、核磁共振氢谱（HNMR）、核磁共振碳谱（CNMR）和元素微量分析等多种技术对所得共聚物进行了表征。采用琼脂扩散法研究了新型鎓盐对大肠杆菌、金黄色葡萄球菌和真菌白色念珠菌、热带念珠菌和近平滑念珠菌的抗菌活性。除了测定所制备化合物的最低抑菌浓度（MIC）外，还根据抑菌圈直径研究了抗菌活性。在受试微生物中，大肠杆菌和金黄色葡萄球菌受影响最大，对于杀生物剂（V）和（VII），抑菌圈为19 - 21（mm）。所得结果表明，季鎓盐的活性高于天冬氨酸共聚物，（VII）和（V）的MIC浓度为25 mg/mL，（VI）和（IV）的MIC浓度为50 mg/mL。