Department of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan 31116, Korea.
Department of Pharmacology, School of Medicine, Eulji University, Daejeon 34824, Korea.
Molecules. 2020 Dec 23;26(1):35. doi: 10.3390/molecules26010035.
species are well known plants distributed throughout the world, and they contain various bioactive components with different biological activities including anti-cancer effects. In this study, we investigated the inhibitory effect of L. (A.S.) extract on cell survival and IL-2-mediated inflammation in human T cell acute lymphocytic leukemia (T-ALL) Jurkat cells. Our results showed that A.S. extract induced caspase-dependent apoptosis of Jurkat cells with no significant cytotoxicity in the normal peripheral blood mononuclear cells. A.S. extract induced ROS generation through the activation of MAPK p38 phosphorylation. It also inhibited IL-2 mRNA expression and NF-κB signaling mediated by phorbol 12-myristate 13-acetate, and phytohemagglutinin. Combined treatment with A.S. extract and axitinib/dovitinib exerted enhanced inhibitory effects on T-ALL cell growth and IL-2 production. These results provide novel information on the potential use of A.S. extract as a therapeutic herbal agent for the treatment and prevention of T-ALL.
物种是分布于世界各地的著名植物,它们含有各种具有不同生物活性的生物活性成分,包括抗癌作用。在这项研究中,我们研究了 L.(A.S.)提取物对人 T 细胞急性淋巴细胞白血病(T-ALL)Jurkat 细胞存活和 IL-2 介导的炎症的抑制作用。我们的结果表明,A.S. 提取物诱导 Jurkat 细胞 caspase 依赖性细胞凋亡,而对正常外周血单个核细胞没有明显的细胞毒性。A.S. 提取物通过激活 MAPK p38 磷酸化诱导 ROS 生成。它还抑制佛波醇 12-肉豆蔻酸 13-乙酸酯和植物血凝素介导的 IL-2 mRNA 表达和 NF-κB 信号。A.S. 提取物与 axitinib/dovitinib 联合治疗对 T-ALL 细胞生长和 IL-2 产生具有增强的抑制作用。这些结果为 A.S. 提取物作为治疗 T-ALL 的治疗性草药制剂的潜在用途提供了新的信息。