Lee Jang Hee, Chung Dae Jun, Lee Je Min, Yeam Inhwa
Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea.
Department of Horticulture and Breeding, Andong National University, Andong 36729, Korea.
Plants (Basel). 2020 Dec 23;10(1):9. doi: 10.3390/plants10010009.
Tomato yellow leaf curl virus (TYLCV) is a disease that is damaging to tomato production worldwide. Resistance to TYLCV has been intensively investigated, and single resistance genes such as have been widely deployed in breeding programs. However, resistance-breaking incidences are frequently reported, and achieving durable resistance against TYLCV in the field is important. In this study, gene-specific markers for and , and closely-linked markers for were developed and applied to distinguish TYLCV resistance in various tomato genotypes. Quantitative infectivity assays using both natural infection in the field and artificial inoculation utilizing infectious TYLCV clones in a growth chamber were optimized and performed to investigate the individual and cumulative levels of resistance. We confirmed that could also be an effective source of resistance for TYLCV control, together with . Improvement of resistance as a result of gene-pyramiding was speculated, and breeding lines including both and showed the strongest resistance in both field and artificial infections.
番茄黄化曲叶病毒（TYLCV）是一种对全球番茄生产造成损害的病害。人们对TYLCV的抗性进行了深入研究，并且诸如[具体基因名称未给出]等单个抗性基因已在育种计划中广泛应用。然而，抗性丧失的情况屡有报道，因此在田间实现对TYLCV的持久抗性至关重要。在本研究中，开发了[具体基因名称未给出]和[具体基因名称未给出]的基因特异性标记，以及[具体基因名称未给出]的紧密连锁标记，并将其应用于区分不同番茄基因型对TYLCV的抗性。利用田间自然感染和在生长室中使用感染性TYLCV克隆进行人工接种的定量感染性测定方法得到了优化并实施，以研究个体抗性水平和累积抗性水平。我们证实，[具体基因名称未给出]与[具体基因名称未给出]一样，也可能是控制TYLCV的有效抗性来源。推测基因聚合可提高抗性，同时包含[具体基因名称未给出]和[具体基因名称未给出]的育种系在田间和人工感染中均表现出最强的抗性。
