Papagiannopoulos Aristeidis, Pippa Natassa, Demetzos Costas, Pispas Stergios, Radulescu Aurel
Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, Greece.
Department of Pharmaceutical Technology, Faculty of Pharmacy, Panepistimioupolis Zografou, National and Kapodistrian University of Athens, 15771 Athens, Greece.
Polymers (Basel). 2020 Dec 22;13(1):4. doi: 10.3390/polym13010004.
The ability of mixtures of 1.2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and the amphiphilic diblock copolymers poly (ethylene oxide)-block-poly(ε-caprolactone) (PEO-b-PCL) to stabilize uni-lamellar nano-vesicles is reported. Small angle neutron scattering (SANS) is used to define their size distribution and bilayer structure and resolve the copresence of aggregates and clusters in solution. The vesicles have a broad size distribution which is compatible with bilayer membranes of relatively low bending stiffness. Their mean diameter increases moderately with temperature and their number density and mass is higher in the case of the diblock copolymer with the larger hydrophobic block. Bayesian analysis is performed in order to justify the use of the particular SANS fitting model and confirm the reliability of the extracted parameters. This study shows that amphiphilic block copolymers can be effectively used to prepare mixed lipid-block copolymer vesicles with controlled lamellarity and a significant potential as nanocarriers for drug delivery.
据报道，1,2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）与两亲性二嵌段共聚物聚环氧乙烷-嵌段-聚己内酯（PEO-b-PCL）的混合物具有稳定单层纳米囊泡的能力。小角中子散射（SANS）用于确定其尺寸分布和双层结构，并解析溶液中聚集体和簇的共存情况。这些囊泡具有较宽的尺寸分布，这与弯曲刚度相对较低的双层膜相符。它们的平均直径随温度适度增加，并且在具有较大疏水嵌段的二嵌段共聚物的情况下，其数量密度和质量更高。进行贝叶斯分析以证明使用特定的SANS拟合模型的合理性，并确认提取参数的可靠性。这项研究表明，两亲性嵌段共聚物可有效地用于制备具有可控层状结构的混合脂质-嵌段共聚物囊泡，并且作为药物递送的纳米载体具有巨大潜力。
