Wang Tse-Yao, Lee Yi-Tzu, Chen Hsien-Yi, Ko Cheng-Hao, Hong Chi-Tsung, Wen Jyun-Wei, Yen Tzung-Hai, Cheng Chao-Min
Department of Emergency Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan.
School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.
Diagnostics (Basel). 2020 Dec 22;11(1):6. doi: 10.3390/diagnostics11010006.
Paraquat is a highly toxic herbicide. Paraquat poisoning is often fatal and is an important public health threat in many places. The quick identification and timely initiation of treatment based on timely analysis of the paraquat concentration in urine/serum could improve the prognosis for patients. However, current paraquat concentration measurements are time-consuming and difficult to implement due to the expensive and bulky equipment required. To address these practical challenges, paper-based devices have emerged as alternative diagnostic tools for improving point-of-care testing. In this study, we demonstrate the successful use of a paper-based analytical device for the accurate detection of urine paraquat concentration. The developed paper-based analytical device employs colorimetric paraquat concentration measurements. The R value for the urine paraquat standard curve was 0.9989, with a dynamic range of 0-100 ppm. The limit of detection was 3.01 ppm. Two other optical-based approaches, Spectrochip and NanoDrop, were used for comparison. The results suggest that the developed paper-based analytical device is comparable to other colorimetric measurements, as determined by Bland-Altman analysis. The device was clinically validated using urine from six paraquat-poisoned patients. The results prove that the developed paper-based analytical device is accurate, easy-to-use, and efficient for urine paraquat concentration measurement, and may enable physicians to improve clinical management.
百草枯是一种剧毒除草剂。百草枯中毒往往是致命的，在许多地方都是一项重大的公共卫生威胁。基于对尿液/血清中百草枯浓度的及时分析进行快速识别并及时开始治疗，可改善患者的预后。然而，由于所需设备昂贵且体积庞大，目前的百草枯浓度测量既耗时又难以实施。为应对这些实际挑战，纸质设备已成为改善即时检测的替代诊断工具。在本研究中，我们展示了一种纸质分析设备成功用于准确检测尿液中百草枯浓度。所开发的纸质分析设备采用比色法测量百草枯浓度。尿液百草枯标准曲线的R值为0.9989，动态范围为0 - 100 ppm。检测限为3.01 ppm。另外两种基于光学的方法，即光谱芯片和微量紫外可见分光光度计，用于比较。结果表明，经布兰德 - 奥特曼分析确定，所开发的纸质分析设备与其他比色测量方法相当。该设备使用六名百草枯中毒患者的尿液进行了临床验证。结果证明，所开发的纸质分析设备在尿液百草枯浓度测量方面准确、易用且高效，可能使医生能够改善临床管理。
