Biochemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Chair for Biomarkers of Chronic Diseases, College of Science, King Saud University, Riyadh 11421, Saudi Arabia.
Dis Markers. 2020 Dec 9;2020:1285216. doi: 10.1155/2020/1285216. eCollection 2020.
The RANKL/RANK/OPG pathway regulates bone remodelling and turnover. However, the genetic background of bone mineral density (BMD) and osteopenia in Saudi postmenopausal women is yet to be studied. We studied the genetic polymorphism of with BMD and other associated factors in Saudi postmenopausal osteopenic women. A total of 439 (223 osteopenia and 216 control) postmenopausal women were recruited from the orthopaedic department of the King Khalid University Hospital, Riyadh, KSA. Genetic variants of (rs1805034 and rs35211496), (rs2277438 and rs9533156), and (rs2073618 and rs3102735) were genotyped using RT-PCR. Anthropometrics, bone mineral density, and other bone markers were measured. The levels of bone turnover markers, PTH, and RANKL were found to be significantly different between control and the osteopenia group. The odds ratio of 2.37 (1.00-5.69) for SNP (rs1805034) indicates that subjects with CC genotype are more vulnerable to developing osteopenia as compared to subjects with TT genotype. Similarly, for SNP (rs2277438), the significant odds ratio of 20.56 (9.82-43.06) indicates that the subjects with GG genotype are at significantly higher risk of having osteopenia compared with the AA genotype subjects. In addition, G allele in rs2277438 also found to be a risk factor for osteopenia 4.54 (3.18-6.49) compared with A allele. However, none of the OPG genotypes shows association with osteopenia. The association of polymorphisms with osteopenia shows its clinical importance in the diagnosis and prognosis of the bone diseases; here, we suggest that the subjects with and polymorphisms may develop osteoporosis.
RANKL/RANK/OPG 通路调节骨重塑和转换。然而，沙特绝经后妇女的骨密度 (BMD) 和骨质疏松的遗传背景尚未得到研究。我们研究了与沙特绝经后骨质疏松女性 BMD 和其他相关因素相关的 基因多态性。总共招募了 439 名（223 名骨质疏松症和 216 名对照组）来自沙特阿拉伯利雅得 King Khalid 大学医院骨科的绝经后妇女。使用 RT-PCR 对 （rs1805034 和 rs35211496）、 （rs2277438 和 rs9533156）和 （rs2073618 和 rs3102735）的遗传变异进行了基因分型。测量了人体测量学、骨矿物质密度和其他骨标志物。发现控制组和骨质疏松组之间的骨转换标志物、PTH 和 RANKL 水平有显著差异。 SNP（rs1805034）的优势比为 2.37（1.00-5.69）表明，与 TT 基因型相比，CC 基因型的个体更容易发生骨质疏松症。同样，对于 SNP（rs2277438），显著的优势比 20.56（9.82-43.06）表明，与 AA 基因型相比，GG 基因型的个体患骨质疏松症的风险显著更高。此外，与 A 等位基因相比，rs2277438 中的 G 等位基因也被发现是骨质疏松症的危险因素 4.54（3.18-6.49）。然而，OPG 基因型均与骨质疏松症无关。 多态性与骨质疏松症的关联表明其在骨骼疾病的诊断和预后中的临床重要性；在这里，我们建议 和 多态性的个体可能会发展为骨质疏松症。
