Sherwin Virginia E, Santi Morena, Walker Olivia, Pickwell Natalie D, Coffey Tracey J, Leigh James A, Egan Sharon A
The School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, UK.
Vet Med Int. 2020 Dec 8;2020:8828624. doi: 10.1155/2020/8828624. eCollection 2020.
is one of the leading causes worldwide of mastitis in the dairy industry, with the most likely sources of infection attributed to environmental reservoirs such as contaminated bedding materials. Early detection of those cases most likely to progress to clinical disease would lead to improved animal welfare, a critical component of overall health and productivity. A multiplex PCR-based diagnostic test was developed for detection of directly from milk and targeting two genes previously identified as important for intramammary colonisation and persistence in dairy cattle. Results indicated the threshold for detection directly from milk was 20,000 CFU/ml and this was achieved without the need for preenrichment. In addition, could be identified from milk samples collected during intramammary challenge studies, prior to clinical signs of infection and at much lower detection limits. The PCR test developed for confirmation of the presence of directly from infected milk has potential value as a diagnostic test to identify early infection and/or to confirm that antibiotic therapy has been successful.
是全球乳制品行业乳腺炎的主要病因之一，最可能的感染源归因于环境储库，如受污染的垫料。早期发现那些最有可能发展为临床疾病的病例将有助于改善动物福利，这是整体健康和生产力的关键组成部分。开发了一种基于多重PCR的诊断测试，用于直接从牛奶中检测，并靶向两个先前被确定对奶牛乳房内定植和持续存在很重要的基因。结果表明，直接从牛奶中检测的阈值为20,000 CFU/ml，无需预富集即可实现。此外，在乳房内攻毒研究期间收集的牛奶样本中，在出现感染临床症状之前且检测限低得多的情况下，可以鉴定出。为直接从受感染牛奶中确认的存在而开发的PCR测试作为一种诊断测试具有潜在价值，可用于识别早期感染和/或确认抗生素治疗是否成功。
