Liu Wenming, Sun Meilin, Han Kai, Hu Rui, Liu Dan, Wang Jinyi
Departments of Biomedical Engineering and Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, China.
Department of Chemistry, College of Chemistry and Pharmacy, Northwest A&F University, Yangling, Shaanxi 712100, China.
ACS Omega. 2020 Dec 11;5(50):32753-32760. doi: 10.1021/acsomega.0c05190. eCollection 2020 Dec 22.
Polydimethylsiloxane (PDMS) is a popular and property-advantageous material for developing biomedical microsystems and advancing cell microengineering. The requirement of constructing a robust cell-adhesive PDMS interface drives the exploration of simple, straightforward, and applicable surface modification methods. Here, a comprehensive evaluation of highly stable neuronal cell adhesion and culture on the PDMS surface modified in one step using functionalized Pluronic is presented. According to multiple comparative tests, this modification is sufficiently verified to enable more significant cell adhesion and spreading in both quantity and stability, higher neuronal differentiation and viability/growth, more complete formation of the neuronal network, and stabler neuronal cell culture than the common coating tools on the PDMS substrate. The comparable and even superior cellular effects of this modification on PDMS to the standard coating of polystyrene for in vitro neurological research are demonstrated. Long-term microfluidic neuron culture with stable adhesion and high differentiation on the modified PDMS interface is accomplished, too. The achievement provides a detailed experimental demonstration of this simple and effective modification for strengthening neuronal cell culture on the PDMS substrate, which is useful for potential applications in the fields of neurobiology, neuron microengineering, and brain-on-a-chip.
聚二甲基硅氧烷（PDMS）是一种用于开发生物医学微系统和推进细胞微工程的常用且具有性能优势的材料。构建坚固的细胞粘附性PDMS界面的需求推动了对简单、直接且适用的表面改性方法的探索。在此，本文对使用功能化普朗尼克一步法改性的PDMS表面上高度稳定的神经元细胞粘附和培养进行了全面评估。根据多项对比测试，与PDMS基底上的常用包被工具相比，这种改性已得到充分验证，能够在数量和稳定性方面实现更显著的细胞粘附和铺展、更高的神经元分化以及活力/生长、更完整的神经网络形成以及更稳定的神经元细胞培养。结果表明，这种对PDMS的改性在体外神经学研究中对细胞产生的效果与聚苯乙烯标准包被相当甚至更优。同时，也实现了在改性的PDMS界面上进行具有稳定粘附和高分化的长期微流控神经元培养。这一成果为这种用于加强PDMS基底上神经元细胞培养的简单有效改性提供了详细的实验证明，对神经生物学、神经元微工程和芯片大脑等领域的潜在应用具有重要意义。
