Bessho-Uehara Manabu, Huang Wentao, Patry Wyatt L, Browne William E, Weng Jing-Ke, Haddock Steven H D
Monterey Bay Aquarium Research Institute, Moss Landing, CA 95039, USA.
Institute for Advanced Research, Nagoya University, Nagoya, Aichi 464-8601 Japan.
iScience. 2020 Dec 10;23(12):101859. doi: 10.1016/j.isci.2020.101859. eCollection 2020 Dec 18.
Coelenterazine is a key substrate involved in marine bioluminescence which is used for light-production by at least nine phyla. Some luminous animals, such as the hydromedusa , lack the ability to produce coelenterazine endogenously and instead depend on dietary sources. Little is known about the source organisms or the metabolic process of coelenterazine biosynthesis. Here, we present evidence that ctenophores are both producers and suppliers of coelenterazine in marine ecosystems. Using biochemical assays and mass spectrometry analyses, we detected coelenterazine from cultured ctenophores fed with a non-luminous coelenterazine-free diet. We propose that ctenophores are an emerging model organism to study coelenterazine biosynthesis and the origins of bioluminescence.
腔肠素是参与海洋生物发光的关键底物，至少有九个门的生物利用它来产生光。一些发光动物，如水螅水母，缺乏内生产生腔肠素的能力，而是依赖于食物来源。关于腔肠素生物合成的来源生物体或代谢过程，人们知之甚少。在这里，我们提供证据表明栉水母是海洋生态系统中腔肠素的生产者和供应者。通过生化分析和质谱分析，我们从喂食不含腔肠素的非发光食物的培养栉水母中检测到了腔肠素。我们认为栉水母是研究腔肠素生物合成和生物发光起源的新兴模式生物。
