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利用成年、无菌. 进行二选一喂养试验的方案

Protocol for a Binary Choice Feeding Assay Using Adult, Axenic .

机构信息

Aix-Marseille Université CNRS, IBDM, Marseille, France.

出版信息

STAR Protoc. 2020 Sep 25;1(3):100117. doi: 10.1016/j.xpro.2020.100117. eCollection 2020 Dec 18.

DOI:10.1016/j.xpro.2020.100117
PMID:33377013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7756975/
Abstract

This protocol is designed to prepare adult axenic before monitoring their behavior in a two-choice feeding assay, where flies are confronted with an axenic versus a dead or alive bacteria-contaminated feeding solution. Several aspects of the procedure, including raising and aging flies in axenic conditions, starving adult flies, and composing feeding solutions, are detailed. The bacterium used in this protocol, ( ), is commonly used to decipher the mechanisms controlling host-pathogen interactions in the model. For complete details on the use and execution of this protocol, please refer to Charroux et al. (2020).

摘要

本方案旨在准备成年无菌果蝇，然后在双选择喂食实验中监测它们的行为，该实验中果蝇面临无菌与死亡或活菌污染的喂食溶液的选择。本方案详细说明了包括在无菌条件下饲养和老化果蝇、使成年果蝇挨饿以及配制喂食溶液等几个方面。本方案中使用的细菌是 （ ），通常用于解析控制 模型中宿主-病原体相互作用的机制。有关此方案使用和执行的完整详细信息，请参见 Charroux 等人（2020 年）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630d/7756975/7e9c506b855d/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630d/7756975/90dff05cac2f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630d/7756975/156923abc1cb/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630d/7756975/e7ebe7e9f1ae/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630d/7756975/08c5abdf67d9/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630d/7756975/7e9c506b855d/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630d/7756975/90dff05cac2f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630d/7756975/156923abc1cb/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630d/7756975/e7ebe7e9f1ae/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630d/7756975/08c5abdf67d9/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/630d/7756975/7e9c506b855d/gr4.jpg

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引用本文的文献

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本文引用的文献

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iScience. 2020 Jun 26;23(6):101152. doi: 10.1016/j.isci.2020.101152. Epub 2020 May 12.
2
Commensal bacteria and essential amino acids control food choice behavior and reproduction.共生细菌和必需氨基酸控制食物选择行为和繁殖。
PLoS Biol. 2017 Apr 25;15(4):e2000862. doi: 10.1371/journal.pbio.2000862. eCollection 2017 Apr.
3
Octopamine mediates starvation-induced hyperactivity in adult Drosophila.
章鱼胺介导成年果蝇饥饿诱导的多动。
Proc Natl Acad Sci U S A. 2015 Apr 21;112(16):5219-24. doi: 10.1073/pnas.1417838112. Epub 2015 Apr 6.
4
NIH Image to ImageJ: 25 years of image analysis.NIH 图像到 ImageJ：25 年的图像分析。
Nat Methods. 2012 Jul;9(7):671-5. doi: 10.1038/nmeth.2089.
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Peptidoglycan sensing by the receptor PGRP-LE in the Drosophila gut induces immune responses to infectious bacteria and tolerance to microbiota.果蝇肠道中受体 PGRP-LE 对肽聚糖的感应可诱导对传染性细菌的免疫反应和对微生物群的耐受。
Cell Host Microbe. 2012 Aug 16;12(2):153-65. doi: 10.1016/j.chom.2012.06.002.
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Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3376-81. doi: 10.1073/pnas.97.7.3376.