将 Stem Cell ASCL2 Reporter STAR 导入肠道类器官。
Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids.
机构信息
Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, University Utrecht, Utrecht, The Netherlands.
Oncode Institute, Utrecht, The Netherlands.
出版信息
STAR Protoc. 2020 Oct 8;1(3):100126. doi: 10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18.
Patient-derived organoids maintain functional and phenotypic characteristics of the original tissue such as cell-type diversity. Here, we provide protocols on how to label intestinal (cancer) stem cells by integrating the stem cell ASCL2 reporter (STAR) into human and mouse genomes via two different strategies: (1) lentiviral transduction or (2) transposon-based integration. Organoid technology, in combination with the user-friendly nature of STAR, will facilitate basic research in human and mouse adult stem cell biology. For complete details on the use and execution of this protocol, please refer to Oost et al. (2018).
类器官保持了原始组织的功能和表型特征，例如细胞类型多样性。在这里，我们提供了通过两种不同策略（1：慢病毒转导或 2：转座子整合）将干细胞 ASCL2 报告基因（STAR）整合到人基因组和小鼠基因组中的方法，来标记肠（癌）干细胞的方案。类器官技术与 STAR 的易用性相结合，将促进人类和小鼠成体干细胞生物学的基础研究。有关此方案的使用和执行的完整详细信息，请参见 Oost 等人。（2018 年）。
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