Department of Biochemistry, McMaster University, Hamilton, ON L8S 4L8, Canada.
Department of Surgery, McMaster University, Hamilton, ON L8S 4L8, Canada.
STAR Protoc. 2020 Nov 21;1(3):100179. doi: 10.1016/j.xpro.2020.100179. eCollection 2020 Dec 18.
Glioblastoma (GBM) remains the most common malignant primary brain tumor in adults with a median survival of less than ~15 months. Further understanding and therapeutic development rely on the use of clinically relevant models of GBM. Here, we present our patient-derived and models that enrich for GBM stem cells (GSCs), a subpopulation of tumor cells with stem cell-like properties that recapitulate the cellular heterogeneity of its parental tumor and resist conventional therapy and seed disease relapse. For complete details on the use and execution of this protocol, please refer to Vora et al. (2020).
胶质母细胞瘤（GBM）仍然是成年人中最常见的恶性原发性脑肿瘤，中位生存期不到~15 个月。进一步的理解和治疗发展依赖于使用临床相关的 GBM 模型。在这里，我们提出了我们的患者来源的 和 模型，这些模型富集了 GBM 干细胞（GSCs），这是肿瘤细胞的一个亚群，具有类似于干细胞的特性，能够重现其亲本肿瘤的细胞异质性，并抵抗传统治疗和引发疾病复发。有关本协议使用和执行的完整详细信息，请参见 Vora 等人。（2020）。
