McMaster Stem Cell and Cancer Research Institute, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada.
Department of Surgery, McMaster University, Hamilton, ON L8S 4L8, Canada.
STAR Protoc. 2020 Dec 2;1(3):100174. doi: 10.1016/j.xpro.2020.100174. eCollection 2020 Dec 18.
Glioblastoma (GBM) is the most common malignant adult brain tumor that is resistant to the standard care therapy. Advances in chimeric antigen receptor (CAR) T cell therapies have spurred renewed interest in developing CAR T cell therapies to target chemoradiotherapy-resistant brain tumor-initiating cells. This protocol shows how to isolate peripheral blood mononuclear cells from healthy donors and generate CAR T cells for the antigens of interest, and how to intracranially inject the CAR T cells into a patient-derived xenograft mouse model of GBM. For complete details on the use and execution of this protocol, please refer to Vora et al. (2020).
胶质母细胞瘤（GBM）是最常见的对标准治疗方法有抗性的成人脑肿瘤。嵌合抗原受体（CAR）T 细胞疗法的进步激发了人们对开发针对化疗和放疗耐药性脑肿瘤起始细胞的 CAR T 细胞疗法的新兴趣。本方案介绍了如何从健康供体中分离外周血单核细胞并生成针对感兴趣抗原的 CAR T 细胞，以及如何将 CAR T 细胞脑内注射到 GBM 患者来源异种移植小鼠模型中。有关该方案使用和实施的完整详细信息，请参见 Vora 等人。（2020 年）。
