肝脂肪变性背景下的肝细胞癌原位种植小鼠模型。
An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis.
机构信息
Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.
Sanford Burnham Prebys Medical Discovery Institute, 10901 N. Torrey Pines Road, La Jolla, CA 92037, USA.
出版信息
STAR Protoc. 2020 Nov 20;1(3):100185. doi: 10.1016/j.xpro.2020.100185. eCollection 2020 Dec 18.
This protocol provides the steps required for a mouse liver orthotopic implantation model. The reliable pre-clinical animal models that have similar characteristics to hepatocellular carcinoma (HCC) are a powerful tool to unveil the mechanisms controlling tumor initiation and progression. Here, we describe a syngeneic orthotopic HCC model that recapitulates the role of a host pro-tumorigenic microenvironment by pre-conditioning mouse livers with a high-fat diet (HFD). For complete details on the use and execution of this protocol, please refer to Kudo et al. (2020).
本方案提供了建立小鼠肝脏原位种植模型所需的步骤。与肝细胞癌（HCC）具有相似特征的可靠临床前动物模型是揭示控制肿瘤起始和进展的机制的有力工具。在这里，我们描述了一种同基因原位 HCC 模型，通过高脂肪饮食（HFD）预处理小鼠肝脏来重现宿主促肿瘤微环境的作用。有关使用和执行本方案的完整详细信息，请参阅 Kudo 等人（2020 年）。
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