Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Department of Pharmacy, Shanghai East Hospital, Tongji University, Shanghai, 200120, China.
STAR Protoc. 2020 Nov 20;1(3):100186. doi: 10.1016/j.xpro.2020.100186. eCollection 2020 Dec 18.
Here, we present a modified protocol for a mouse heart failure (HF) model using minimally invasive transverse aortic constriction (miTAC). miTAC is a more effective method in mice than the standard open-chest transverse aortic constriction (TAC) to generate an HF model. miTAC does not require the cutting of the ribs or tracheal intubation with artificial ventilation; it also has a higher survival rate. The successful outcome of the HF model can be verified using transthoracic echocardiography and histology. For complete details on the use and execution of this protocol, please refer to Hu et al. (2003) and Richards et al. (2019).
在这里，我们提出了一种使用微创主动脉缩窄（miTAC）的小鼠心力衰竭（HF）模型的改良方案。miTAC 是一种比标准开胸主动脉缩窄（TAC）更有效的方法，可用于产生 HF 模型。miTAC 不需要切割肋骨或进行气管插管和人工通气；它的存活率也更高。HF 模型的成功结果可以通过经胸超声心动图和组织学来验证。有关此方案的使用和执行的完整详细信息，请参见 Hu 等人（2003 年）和 Richards 等人（2019 年）的研究。
