Institute for Immunology, Tsinghua University, Beijing 100084, China.
Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, China.
STAR Protoc. 2020 Dec 14;1(3):100218. doi: 10.1016/j.xpro.2020.100218. eCollection 2020 Dec 18.
is an extracellular enteric bacterial pathogen that induces both innate and adaptive immunity in mice, its natural host. Here, we detail the step-by-step procedure to evaluate the immune responses in a mouse model of infection. We describe the methods to establish infection, isolate group 3 innate lymphoid cells from lamina propria lymphocytes, and analyze their response. We also assess the response of T follicular helper cells and germinal center B cells. For complete details on the use and execution of this protocol, please refer to Guo et al. (2015), Kennedy and Hartland, (2018), and Wang et al. (2020).
是一种胞外肠道细菌病原体，可诱导其天然宿主小鼠产生先天和适应性免疫。在此，我们详细介绍了在 感染的小鼠模型中评估免疫反应的逐步程序。我们描述了建立感染、从固有层淋巴细胞中分离第 3 组先天淋巴细胞以及分析其反应的方法。我们还评估了滤泡辅助 T 细胞和生发中心 B 细胞的反应。有关该方案使用和执行的完整详细信息，请参见 Guo 等人（2015 年）、Kennedy 和 Hartland（2018 年）和 Wang 等人（2020 年）。
