柠檬酸杆菌和不动杆菌分别与不同胎龄早产儿的喂养不耐受有关：一项前瞻性病例对照研究。
Citrobacter and Acinetobacter are respectively involved in feeding intolerance in preterm infants of different gestational ages: a prospective case-control study.
作者信息
Fu Chunyan, Xu Jinglin, Wang He, Chen Dongmei, Liu Zhiyong
机构信息
The Graduate School of Fujian Medical University, Fuzhou, 35000, Fujian, China.
Department of Neonatology, Quanzhou Maternity and Children's Hospital, 700 Fengze Street, Quanzhou, 362000, Fujian Province, China.
出版信息
Ital J Pediatr. 2025 Jun 9;51(1):184. doi: 10.1186/s13052-025-02034-3.
BACKGROUND
Feeding intolerance (FI) is a common feeding problem in preterm infants. The gut microbiota contributes significantly to its onset, progression, and outcome. In this study, we aimed to understand the differences in gut microbiota among preterm infants with FI of different gestational ages. The goal was to provide a basis for early probiotic intervention.
METHODS
We undertook a prospective case-control study in which we enrolled 80 preterm infants at a gestational age < 34 weeks. Participants were divided into four groups of 20 each: early preterm infants with FI (EFI group, gestational age < 32 weeks), early preterm infants with feeding tolerance (FT) (EFT group, gestational age < 32 weeks), moderate preterm infants with FI (MFI group, gestational age ≥ 32 weeks), moderate preterm infants with FT (MFT group, gestational age ≥ 32 weeks). 16 S rDNA high-throughput sequencing was employed to analyze the infants' fecal microbiota and examine the potential link between gut microbiota and gestational age. Statistical analysis was conducted for the collected data. The Statistical Package for Social Sciences software was used. T-tests or non-parametric tests were performed for comparison between groups of measurement data, and the χ2 test was used to compare between groups of count data. At the genus and species level, the potential association between intestinal microbiota and FI and the relationship with gestational age were explored.
RESULTS
The abundance of Citrobacter in the feces of the EFI group was significantly higher than that in the EFT group. Additionally, the abundance of Acinetobacter in the MFI group was significantly higher than that in the MFT group. The abundance of Clostridium XI was significantly low in the MFT group.
CONCLUSIONS
Citrobacter and Acinetobacter genera are implicated in FI in preterm infants with gestational ages < 32 weeks and ≥ 32 weeks, respectively. However, Clostridium XI may be involved in regulating intestinal homeostasis in those with a gestational age ≥ 32 weeks.
TRIAL REGISTRATION
ChiCTR, ChiCTR2400086000. Registered 24 June 2024, https://www.chictr.org.cn/showprojEN.html?proj=210,126 .
背景
喂养不耐受（FI）是早产儿常见的喂养问题。肠道微生物群对其发生、发展及结局有重要影响。本研究旨在了解不同胎龄的FI早产儿肠道微生物群的差异，为早期益生菌干预提供依据。
方法
我们进行了一项前瞻性病例对照研究，纳入80例胎龄<34周的早产儿。参与者分为四组，每组20例：早期FI早产儿（EFI组，胎龄<32周）、早期喂养耐受（FT）早产儿（EFT组，胎龄<32周）、中期FI早产儿（MFI组，胎龄≥32周）、中期FT早产儿（MFT组，胎龄≥32周）。采用16S rDNA高通量测序分析婴儿粪便微生物群，探讨肠道微生物群与胎龄之间的潜在联系。对收集的数据进行统计分析。使用社会科学统计软件包。对计量资料组间比较采用t检验或非参数检验，计数资料组间比较采用χ2检验。在属和种水平上，探讨肠道微生物群与FI之间的潜在关联以及与胎龄的关系。
结果
EFI组粪便中柠檬酸杆菌的丰度显著高于EFT组。此外，MFI组不动杆菌的丰度显著高于MFT组。MFT组XI型梭菌的丰度显著降低。
结论
柠檬酸杆菌属和不动杆菌属分别与胎龄<32周和≥32周的早产儿的FI有关。然而，XI型梭菌可能参与调节胎龄≥32周者的肠道稳态。
试验注册
中国临床试验注册中心，ChiCTR2400086000。2024年6月24日注册，https://www.chictr.org.cn/showprojEN.html?proj=210,126 。
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