Zhang Lei, Yu Xu-Hong, Yao Li-Ping
Yantai Stomatological Hospital, Yantai 264008, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2020 Dec 1;38(6):637-641. doi: 10.7518/hxkq.2020.06.005.
To compare the anti-caries effect and safety of Er:YAG laser combined with fluoride and methylene blue-photodynamic therapy (MB-PDT).
A total of 28 rat dental caries models were established and randomly divided into seven groups: photodynamic therapy (PDT) group, laser combined with fluoride group, laser group, sodium fluoride group, and 0.9% saline control group. Spectrophotometric optical density was used to reflect the growth of Streptococcus mutans. Laser-induced fluorescence diagnostic (LF) instrument was utilized to detect the demineralization degree of dental caries. Histopathological sections were employed to observe the damage of dental pulp and buccal mucosa.
The optical density (OD) value of the PDT and combination groups was significantly lower than that of other treatment groups (P<0.05). An increase in LF value and demineralization occurred in varying degrees with different treatment methods. Histopathological observation showed that pulp and buccal mucosa injury was more obvious in the combination group of 70 mw·cm⁻² and Er:YAG laser group compared with other groups.
Under the same parameters, the combined group and PDT have good germicidal efficacy, but PDT has fewer adverse reactions and less damage. It is an effective and safe method for caries prevention.
比较铒激光联合氟化物与亚甲蓝光动力疗法（MB-PDT）的防龋效果及安全性。
共建立28个大鼠龋齿模型，随机分为七组：光动力疗法（PDT）组、激光联合氟化物组、激光组、氟化钠组和0.9%生理盐水对照组。采用分光光度法光密度反映变形链球菌的生长情况。利用激光诱导荧光诊断（LF）仪检测龋齿的脱矿程度。采用组织病理学切片观察牙髓和颊黏膜的损伤情况。
PDT组和联合组的光密度（OD）值显著低于其他治疗组（P<0.05）。不同治疗方法使LF值和脱矿程度有不同程度增加。组织病理学观察显示，与其他组相比，70 mw·cm⁻²联合组和铒激光组的牙髓和颊黏膜损伤更明显。
在相同参数下，联合组和PDT组具有良好的杀菌效果，但PDT组不良反应较少且损伤较小。它是一种有效且安全的防龋方法。
