Liu Jian-Guo, Zhao Hong-le, Li Jiao-Jiao, Ma Ya-Ling, Zhao Yu-Tao
Shanxi Provincial Hospital of Traditional Chinese Medicine, Xi'an, Shaanxi 710003, China.
Shanxi University of Chinese Medicine, Xianyang, Shaanxi 712046, China.
Zhonghua Nan Ke Xue. 2020 Sep;26(9):826-831.
To investigate the effect of Bushen Huoxue Recipe (BHR) on cyclophosphamide-induced apoptosis of testicular spermatogenic cells in mice and its possible action mechanisms.
Fifty male Babl/c mice aged 8－9 weeks were randomly divided into five groups of an equal number: blank control, model control, low-dose BHR, medium-dose BHR and high-dose BHR. The animals in the blank control group were intraperitoneally injected with normal saline, while those in the other four groups with cyclophosphamide at 50 mg/kg/d, all for 7 days. After modeling, the mice in the blank and model control groups were given distilled water via gavage once a day, and those in the low-, medium- and high-dose BHR groups treated intragastrically with BHR at 7.5, 15 and 30 g/kg/d qd for 30 successive days. Then, the apoptosis index of the testicular spermatogenic cells was obtained by TUNEL and the expressions of Bax and Bcl-2 mRNA and proteins determined by RT-PCR and Western blot, respectively.
Compared with the mice in the blank control group, the BHR model controls showed dramatically increased apoptosis of testicular spermatogenic cells and up-regulated mRNA and protein expressions of Bax and Bcl-2 in the testis tissue (P < 0.01). In comparison with the model controls, the mice in the BHR treatment groups exhibited significantly reduced apoptosis of testicular spermatogenic cells and down-regulated mRNA and protein expressions of Bax and Bcl-2 in the testis tissue (P < 0.01).
Bushen Huoxue Recipe can reduce cyclophosphamide-induced apoptosis of testicular spermatogenic cells in mice, which may be associated with its ability of regulating the expressions of Bax and Bcl-2 mRNA and proteins in the testis tissue.
探讨补肾活血方（BHR）对环磷酰胺诱导的小鼠睾丸生精细胞凋亡的影响及其可能的作用机制。
将50只8 - 9周龄的雄性Babl/c小鼠随机分为数量相等的五组：空白对照组、模型对照组、低剂量BHR组、中剂量BHR组和高剂量BHR组。空白对照组小鼠腹腔注射生理盐水，其他四组小鼠腹腔注射环磷酰胺50 mg/kg/d，连续7天。造模后，空白对照组和模型对照组小鼠每天经口灌胃蒸馏水，低、中、高剂量BHR组小鼠分别以7.5、15、30 g/kg/d的剂量连续30天经口灌胃BHR。然后，通过TUNEL法检测睾丸生精细胞的凋亡指数，分别采用RT-PCR和Western blot法检测睾丸组织中Bax和Bcl-2 mRNA及蛋白的表达。
与空白对照组小鼠相比，模型对照组小鼠睾丸生精细胞凋亡显著增加，睾丸组织中Bax和Bcl-2的mRNA及蛋白表达上调（P < 0.01）。与模型对照组相比，BHR治疗组小鼠睾丸生精细胞凋亡显著减少，睾丸组织中Bax和Bcl-2的mRNA及蛋白表达下调（P < 0.01）。
补肾活血方可减少环磷酰胺诱导的小鼠睾丸生精细胞凋亡，这可能与其调节睾丸组织中Bax和Bcl-2 mRNA及蛋白表达的能力有关。
