Song Kun-Kun, Ma Wen-Wen, Huang Cong, Ding Jia-Hui, Cui Dan-Dan, Tan Xiu-Juan, Xiao Jing, Zhang Ming-Min
Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2016 Aug;36(4):571-575. doi: 10.1007/s11596-016-1627-2. Epub 2016 Jul 28.
The aim of the present study was to explore the effect and mechanism of Bushen Huoxue recipe (BHR) on ovarian reserve in mice with premature ovarian failure (POF). Mice were divided into 3 groups: normal group, model group and BHR group. Intraperitoneal injection of cyclophosphamide was performed to create the POF model. Primordial follicular (PDF) number, ovarian wet weight, ovarian index, and estrous cycle were analyzed to evaluate the effect of BHR on POF. Meanwhile, the mRNA and protein level of Mouse Vasa Homologue (MVH) in the bone marrow, peripheral blood and ovary were detected, to explore the underlying mechanism of the treatment efficacy of BHR on ovarian reserve. By the time of BHR treatment for 28 days, BHR increased the PDF number and shortened the estrous cycle of POF mice. BHR also decreased the mRNA level of MVH in the bone marrow, and increased mRNA and protein level of MVH in the ovary of POF mice. Our results demonstrated a treatment efficacy of BHR on POF mice, and revealed that BHR might repair the dysfunction of germline stem cells in the bone marrow, and thus to improve the ovarian reserve and enhance the ovarian function of POF mice through neo-oogenesis.
本研究旨在探讨补肾活血方（BHR）对卵巢早衰（POF）小鼠卵巢储备功能的影响及机制。将小鼠分为3组：正常组、模型组和BHR组。通过腹腔注射环磷酰胺建立POF模型。分析原始卵泡（PDF）数量、卵巢湿重、卵巢指数和动情周期，以评估BHR对POF的影响。同时，检测骨髓、外周血和卵巢中鼠同源血管生成素（MVH）的mRNA和蛋白水平，以探讨BHR对卵巢储备功能治疗效果的潜在机制。在BHR治疗28天时，BHR增加了POF小鼠的PDF数量并缩短了动情周期。BHR还降低了骨髓中MVH的mRNA水平，并增加了POF小鼠卵巢中MVH的mRNA和蛋白水平。我们的结果证明了BHR对POF小鼠的治疗效果，并揭示BHR可能修复骨髓中生殖系干细胞的功能障碍，从而通过新卵泡生成改善POF小鼠的卵巢储备并增强其卵巢功能。
