Yao Zhi-Feng, Pan Zhi-Yao, Yao Yi-Wen, Chen Jin-Fei
Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, China.
Department of Radiotherapy, Medical Center of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210011, China.
Zhonghua Nan Ke Xue. 2020 Jul;26(7):588-594.
To investigate the expression of Linc00662 in PCa and its influence on the biological function of PCa cells.
Using qRT-PCR, we detected the expression of Linc00662 in the PCa tissue and cell lines and that in the adjacent normal prostatic tissue and epithelial WPMY-1 cells and analyzed the correlation between the expression of Linc00662 and the clinicopathological features of the PCa tissue. We transfected PC-3 and DU145 cells with siRNA, and verified the interference efficiency by qRT-PCR. We examined the effects of interfering with the Linc00662 expression on the proliferation, apoptosis, migration and invasiveness of PC-3 and DU145 cells by CCK-8 assay, Caspase 3/9 activity assay, wound-healing assay and Transwell invasion assay.
The expression of Linc00662 in the PCa tissue and cell lines was significantly up-regulated compared with that in the adjacent normal prostatic tissue and epithelial cells (P < 0.01), and the high expression of Linc00662 was positively correlated with the tumor stage (P = 0.002), primary tumor size (P = 0.006), lymph node metastasis (P = 0.001) and distant metastasis (P = 0.001). Transfection of si-Linc00662 into the PC-3 and DU145 cells significantly reduced the expression of Linc00662 (P < 0.01). Compared with the normal control, the PC-3 and DU145 cells in the Linc00662 interference group showed remarkably decreased proliferation, invasion and migration abilities (P < 0.01), but an increased rate of apoptosis (P < 0.01).
Linc00662 is highly expressed in PCa tissues and cells relatively. Knockdown of the Linc00662 expression may inhibit the proliferation, migration and invasiveness and promote the apoptosis of PCa cells. Therefore, Linc00662 could be considered as a new marker of PCa.
探讨Linc00662在前列腺癌（PCa）中的表达及其对PCa细胞生物学功能的影响。
采用qRT-PCR检测Linc00662在PCa组织及细胞系、相邻正常前列腺组织及上皮WPMY-1细胞中的表达，并分析Linc00662表达与PCa组织临床病理特征的相关性。用siRNA转染PC-3和DU145细胞，通过qRT-PCR验证干扰效率。采用CCK-8法、Caspase 3/9活性检测法、划痕愈合试验和Transwell侵袭试验检测干扰Linc00662表达对PC-3和DU145细胞增殖、凋亡、迁移和侵袭能力的影响。
与相邻正常前列腺组织及上皮细胞相比，Linc00662在PCa组织及细胞系中的表达显著上调（P < 0.01），且Linc00662高表达与肿瘤分期（P = 0.002）、原发肿瘤大小（P = 0.006）、淋巴结转移（P = 0.001）和远处转移（P = 0.001）呈正相关。将si-Linc00662转染至PC-3和DU145细胞中可显著降低Linc00662的表达（P < 0.01）。与正常对照组相比，Linc00662干扰组的PC-3和DU145细胞增殖、侵袭和迁移能力明显降低（P < 0.01），但凋亡率升高（P < 0.01）。
Linc00662在PCa组织和细胞中相对高表达。敲低Linc00662表达可能抑制PCa细胞的增殖、迁移和侵袭，并促进其凋亡。因此，Linc00662可被视为PCa的一个新标志物。
