Xu Bin, Chen Shu-Qiu, Liu Ning, Lu Kai, Wang Yi-Duo, Sun Chao, Jiang Hua, Yang Yu, Zhang Xiao-Wen, Xu Bin, Liu Jing, Zhu Wei-Dong, Chen Ming
Department of Urology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu 210009, China.
Zhonghua Nan Ke Xue. 2016 Dec;22(12):1083-1087.
To explore the expression of long noncoding RNA (lncRNA) LINC01358 in prostate cancer (PCa) and its effect on the proliferation and migration of PCa cells.
The lncRNA array was used to screen differentially expressed lncRNAs in PCa and the corresponding carcinoma-adjacent normal tissues from 3 patients. The expressions of LINC01358 in the primary PCa, metastatic PCa, and carcinoma-adjacent tissues were compared using the PCa dataset of the Memorial Sloan Kettering Cancer Center (MSKCC). The data obtained were validated by determining the expression of LINC01358 in the PCa and carcinoma-adjacent tissues of another 10 patients by quantitative real time PCR (qRT-PCR). The effects of lncRNA LINC01358 on the proliferation of DU145 cells and migration of PCa cells were detected by MTT and Transwell assay, respectively.
Totally, 79 differentially expressed lncRNAs in the lncRNA array, 36 highly and the other 43 lowly expressed in the PCa tissue. LINC01358 was up-regulated in the cancerous tissue. According to the MSKCC data, the LINC01358 expression was markedly higher in metastatic PCa (5.81±0.19, n = 19) and primary PCa (5.47±0.04, n = 131) than in the PCa-adjacent tissue (5.15±0.07, n = 29) and significantly correlated with postoperative biochemical relapse of the malignancy (P<0.05). qRT-PCR indicated a remarkably higher expression of LINC01358 in the PCa than in the carcinoma-adjacent tissue (6.02±1.12 vs 3.21±0.21, P<0.05). Transfection of the DU145 cells with siRNA significantly decreased the level of LINC01358 and inhibited the proliferation and migration of the PCa cells.
LINC01358 is highly expressed in the PCa tissue and knockdown of LINC01358 may inhibit the proliferation and migration of PCa cells. LncRNA LINC01358 may be involved in the development and progression of PCa and become an index for the early diagnosis as well as a new target for the gene therapy of the malignancy.
探讨长链非编码RNA（lncRNA）LINC01358在前列腺癌（PCa）中的表达及其对PCa细胞增殖和迁移的影响。
采用lncRNA芯片筛选3例PCa患者及其相应癌旁正常组织中差异表达的lncRNAs。利用纪念斯隆凯特琳癌症中心（MSKCC）的PCa数据集比较LINC01358在原发性PCa、转移性PCa及癌旁组织中的表达。通过定量实时PCR（qRT-PCR）检测另外10例患者PCa及癌旁组织中LINC01358的表达，对所得数据进行验证。分别采用MTT法和Transwell法检测lncRNA LINC01358对DU145细胞增殖及PCa细胞迁移的影响。
lncRNA芯片共筛选出79个差异表达的lncRNAs，其中36个在PCa组织中高表达，43个低表达。LINC01358在癌组织中上调。根据MSKCC数据，LINC01358在转移性PCa（5.81±0.19，n = 19）和原发性PCa（5.47±0.04，n = 131）中的表达明显高于癌旁组织（5.15±0.07，n = 29），且与恶性肿瘤术后生化复发显著相关（P<0.05）。qRT-PCR结果显示，PCa中LINC01358的表达明显高于癌旁组织（6.02±1.12 vs 3.21±0.21，P<0.05）。用小干扰RNA（siRNA）转染DU145细胞可显著降低LINC01358水平，并抑制PCa细胞的增殖和迁移。
LINC01358在PCa组织中高表达，敲低LINC01358可能抑制PCa细胞的增殖和迁移。lncRNA LINC01358可能参与PCa的发生发展，有望成为早期诊断指标及恶性肿瘤基因治疗的新靶点。
