miR-216a 通过抑制 YBX1 抑制弥漫大 B 细胞淋巴瘤的增殖和侵袭。

Inhibition of YBX1 by miR-216a Suppresses Proliferation and Invasion of Diffuse Large B-Cell Lymphoma.

机构信息

Clinical Laboratory, Liaoning Cancer Hospital - Institute, Shenyang City, Liaoning Province, China.

Department of Hematology, Affiliated Hospital of Nantong University, Nantong City, Jiangsu Province, China.

出版信息

Balkan Med J. 2021 May;38(3):171-176. doi: 10.4274/balkanmedj.galenos.2020.2020.8-23.

BACKGROUND

MicroRNAs (miRNAs) could be implicated in tumorigenesis of diffuse large B-cell lymphoma (DLBCL).

AIMS

To determine the role of MiR-216a in DLBCL.

STUDY DESIGN

Cell culture study.

METHODS

Expression of miR-216a in DLBCL cells was examined by qRT-PCR. Cell counting kit-8, bromodeoxyuridine staining and transwell assays were performed to evaluate role of miR-216a on DLBCL cell growth. Target gene of miR-216a was verified by luciferase reporter assay.

RESULTS

MiR-216a was dramatically reduced in DLBCL cells compared to the normal B-cell line (P < .01). MiR-216a reduced the viability and retarded DLBCL cell proliferation. The invasion of DLBCL was suppressed by miR-216a. Y box binding protein 1 (YBX1) was validated as a target of miR-216a. Its expression was reduced by miR-216a mimic and enhanced by miR-216a inhibitor in DB and SU-DHL-10 cells. Knockdown of YBX1 reduced cell viability, proliferation, and invasion of DB and SU-DHL-10 cells.

CONCLUSION

MiR-216a exerted tumor-suppressive effects on DLBCL cells through inhibition of YBX1, providing a new strategy for DLBCL.

背景

微小 RNA（miRNAs）可能与弥漫性大 B 细胞淋巴瘤（DLBCL）的肿瘤发生有关。

目的

确定 MiR-216a 在 DLBCL 中的作用。

研究设计

细胞培养研究。

方法

通过 qRT-PCR 检测 DLBCL 细胞中 miR-216a 的表达。通过细胞计数试剂盒-8、溴脱氧尿苷染色和 Transwell 测定来评估 miR-216a 对 DLBCL 细胞生长的作用。通过荧光素酶报告测定验证 miR-216a 的靶基因。

结果

与正常 B 细胞系相比，DLBCL 细胞中的 miR-216a 显著降低（P<0.01）。MiR-216a 降低了 DLBCL 细胞的活力并延缓了其增殖。MiR-216a 抑制了 DLBCL 的侵袭。Y 盒结合蛋白 1（YBX1）被验证为 miR-216a 的靶基因。miR-216a 模拟物降低了 DB 和 SU-DHL-10 细胞中 YBX1 的表达，而 miR-216a 抑制剂则增强了其表达。DB 和 SU-DHL-10 细胞中 YBX1 的敲低降低了细胞活力、增殖和侵袭。