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长链非编码RNA PVT1对miR-216a-5p的下调通过调节YBX1表达抑制结直肠癌进展。

Downregulation of miR-216a-5p by long noncoding RNA PVT1 suppresses colorectal cancer progression via modulation of YBX1 expression.

作者信息

Zeng Xiang, Liu Yan, Zhu Hongquan, Chen Di, Hu Weimin

机构信息

Gastrointesitinal Oncosurgery, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, People's Republic of China.

Clinical Laboratory, Guangdong Women's and Children's Hospital, Guangzhou, People's Republic of China.

出版信息

Cancer Manag Res. 2019 Jul 24;11:6981-6993. doi: 10.2147/CMAR.S208983. eCollection 2019.

DOI:10.2147/CMAR.S208983
PMID:31440087
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6664428/
Abstract

PURPOSE

Increasing evidence has demonstrated that microRNAs (miRNAs) are closely related to the occurrence and development of tumors. MiR-216a-5p, located at 2p16.1, has been shown to suppress proliferation of cancerous cells. However, its expression and function in colorectal cancer (CRC) remain unclear.

MATERIALS AND METHODS

The significance of miR-216a-5p in CRC was studied by analyzing miR-216a-5p expression in CRC tissues and its association with clinicopathological parameters. CRC cells, stably overexpressing miR-216a-5p, were evaluated for cell proliferation and metastasis using cell counting kit-8 (CCK-8) and transwell assay methods. Epithelial-mesenchymal transition (EMT) pathway was analyzed by Western blotting. Bioinformatics, quantitative real-time polymerase chain reaction (RT-qPCR), and luciferase reporter assay were performed to define the regulation of PVT1/miR-216a-5p/Y Box Binding Protein 1 (YBX1) axis in CRC.

RESULTS

The expression of miR-216a-5p was found to be significantly downregulated in CRC and was correlated with the various stages and differentiation degree of the tumors. Moreover, the overexpression of miR-216a-5p could significantly inhibit the tumor growth, metastasis, and EMT progression in CRC. Furthermore, the expression of miR-216a-5p was negatively correlated with the expression of PVT1, and PVT1 could reverse tumor suppressive effect of miR-216a-5p in CRC cells. Finally, YBX1 might be the key target of PVT1/miR-216a-5p axis in CRC.

CONCLUSION

Downregulation of miR-216a-5p by PVT1 could suppress CRC progression via modulating YBX1 expression.

摘要

目的

越来越多的证据表明，微小RNA（miRNA）与肿瘤的发生和发展密切相关。位于2p16.1的miR-216a-5p已被证明可抑制癌细胞的增殖。然而，其在结直肠癌（CRC）中的表达和功能仍不清楚。

材料与方法

通过分析CRC组织中miR-216a-5p的表达及其与临床病理参数的关系，研究miR-216a-5p在CRC中的意义。使用细胞计数试剂盒-8（CCK-8）和Transwell检测方法，对稳定过表达miR-216a-5p的CRC细胞进行细胞增殖和转移评估。通过蛋白质免疫印迹分析上皮-间质转化（EMT）途径。进行生物信息学、定量实时聚合酶链反应（RT-qPCR）和荧光素酶报告基因检测，以确定PVT1/miR-216a-5p/Y盒结合蛋白1（YBX1）轴在CRC中的调控作用。

结果

发现miR-216a-5p在CRC中的表达显著下调，且与肿瘤的不同阶段和分化程度相关。此外，miR-216a-5p的过表达可显著抑制CRC中的肿瘤生长、转移和EMT进程。此外，miR-216a-5p的表达与PVT1的表达呈负相关，且PVT1可逆转miR-2

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/337ab07f8de1/CMAR-11-6981-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/854babda1864/CMAR-11-6981-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/03374f827541/CMAR-11-6981-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/fcea0fbafa82/CMAR-11-6981-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/dd972d5f5b22/CMAR-11-6981-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/e0ec1fc35d66/CMAR-11-6981-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/337ab07f8de1/CMAR-11-6981-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/854babda1864/CMAR-11-6981-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/03374f827541/CMAR-11-6981-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/fcea0fbafa82/CMAR-11-6981-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/dd972d5f5b22/CMAR-11-6981-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/e0ec1fc35d66/CMAR-11-6981-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d658/6664428/337ab07f8de1/CMAR-11-6981-g0006.jpg

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