Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
ACS Appl Mater Interfaces. 2021 Jan 13;13(1):1914-1920. doi: 10.1021/acsami.0c18280. Epub 2020 Dec 30.
Graphdiyne (GD) is a new kind of carbon nanomaterial which has carbon-carbon triple bonds to form a layered structure. Here, we report the application of GD as the matrix for small molecule analysis in laser desorption ionization mass spectrometry (LDI MS). The GD matrix displayed two advantages: little background in the low mass range and good molecular ion signal in negative ion mode for many small molecules, e.g., fatty acids, amino acids, peptides, and drugs can be obtained in negative ion mode. By comparing the signal intensity of tetraphenylborate and juglone with and without GD existing, it was found that GD can enhance both of the desorption efficiency and ionization efficiency in LDI. Through analysis of the serum samples from liver cancer patients and healthy people, the GD-assisted LDI MS results showed that fatty acids could be used as potential biomarkers for the early diagnosis of liver cancer.
石墨炔（GD）是一种新型的碳纳米材料，具有碳-碳三键，形成层状结构。在这里，我们报告了 GD 作为基质在激光解吸电离质谱（LDI MS）中小分子分析中的应用。GD 基质表现出两个优点：在低质量范围内背景较小，并且对于许多小分子（例如脂肪酸、氨基酸、肽和药物）在负离子模式下具有良好的分子离子信号。通过比较存在和不存在 GD 时四苯硼酸盐和胡桃醌的信号强度，发现 GD 可以提高 LDI 中的解吸效率和离子化效率。通过对肝癌患者和健康人血清样本的分析，GD 辅助的 LDI MS 结果表明，脂肪酸可以作为肝癌早期诊断的潜在生物标志物。
