Behera Ipsita Dipamitra, Basak Geetanjali, Kumar Ravi Ranjan, Sen Ramkrishna, Meikap Bhim Charan
Department of Chemical Engineering, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India.
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2021;56(2):226-239. doi: 10.1080/10934529.2020.1866924. Epub 2020 Dec 30.
Petroleum hydrocarbons (PHCs) in petroleum refinery sludge (PRS) are the most adverse components because of their toxic nature, which are harmful to human health and the aquatic ecosystem. This study aimed to identify and characterize an indigenous bacterium isolated from PRS of Indian oil corporation ltd. (IOCL), Haldia, India, and evaluate its performance for biodegradation of total petroleum hydrocarbon (TPH) of PRS. The bacterium molecularly characterized as sp. IRB19 by 16S rRNA sequencing and phylogenetic analysis. The strain IRB19 showed a significant ability to utilize four different oils (kerosene, diesel, petrol and hexadecane) . IRB19 could able to degrade up to 65 ± 2.4% of TPH in 28 d of incubation. Solvent extraction study showed that PRS contain 180.57 ± 3.44 g kg of TPH and maltene fraction composed of aliphatic, aromatics and polar components of 52 ± 4, 39 ± 2 and 9 ± 1%, respectively. The TPH degradation best fitted for the Gompertz model and followed the first-order kinetics having the rate constant () and half-life period ( ) of 0.036 d and 19 d, respectively. Results of this study verified the suitability of the novel strain IRB19 for the biodegradation of PHCs.
炼油厂污泥（PRS）中的石油烃（PHCs）因其毒性是最有害的成分，对人类健康和水生生态系统都有危害。本研究旨在鉴定和表征从印度石油公司（IOCL）位于印度哈尔迪亚的炼油厂污泥中分离出的一种本土细菌，并评估其对炼油厂污泥中总石油烃（TPH）的生物降解性能。通过16S rRNA测序和系统发育分析，该细菌在分子水平上被鉴定为 sp. IRB19。菌株IRB19显示出利用四种不同油品（煤油、柴油、汽油和十六烷）的显著能力。在28天的培养期内，IRB19能够降解高达65±2.4%的TPH。溶剂萃取研究表明，炼油厂污泥中含有180.57±3.44 g/kg的TPH，其中软沥青馏分分别由52±4%的脂肪族、39±2%的芳香族和9±1%的极性成分组成。TPH降解最符合Gompertz模型，遵循一级动力学，速率常数（）和半衰期（）分别为0.036 d和19 d。本研究结果证实了新型菌株IRB19对石油烃生物降解的适用性。
