Wu Shasha, Ma Xiaoyan, Wang Yujing, Zhou Jie, Li Xianghua, Wang Xiaobo
School of Pharmacy, Hubei University of Science and Technology, Xianning 437100, PR China.
Provincial Key Laboratory of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, 241000, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Mar 15;249:119330. doi: 10.1016/j.saa.2020.119330. Epub 2020 Dec 14.
A new BODIPY-based fluorescent probe (BC-DPA) was prepared by a simple method for Cu detection in aqueous media and living cells. BC-DPA displayed excellent selectivity toward Cuvia fluorescence "turn-off" mode when a mononuclear Cu(Ⅱ) complex is formed. The corresponding BC-DPA-Cu(Ⅱ) complex, whose structure was characterized by X-ray crystallography, has Cu(Ⅱ) in a distorted octahedral geometry. On the basis of the displacement approach, the fluorescence of BC-DPA-Cu was recovered in the presence of S, which allowed the system to act as a sensitive "turn-on" sensor for hydrogen sulfide. Furthermore, BC-DPA exhibited noticeable permeability and low cytotoxicity, making it a useful tool to detect Cu in biosystems.
通过一种简单的方法制备了一种基于BODIPY的新型荧光探针（BC-DPA），用于在水性介质和活细胞中检测铜。当形成单核Cu(Ⅱ)配合物时，BC-DPA对Cu呈现出优异的选择性，荧光呈“关断”模式。通过X射线晶体学对其结构进行表征的相应BC-DPA-Cu(Ⅱ)配合物中，Cu(Ⅱ)具有扭曲的八面体几何构型。基于置换法，在硫存在的情况下，BC-DPA-Cu的荧光得以恢复，这使得该体系能够作为一种灵敏的硫化氢“开启”传感器。此外，BC-DPA表现出显著的渗透性和低细胞毒性，使其成为生物系统中检测铜的有用工具。
