Chen Lili, Tan Hongliang, Xu Fugang, Wang Li
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Province, People's Republic of China.
College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, People's Republic of China.
Luminescence. 2018 Feb;33(1):161-167. doi: 10.1002/bio.3386. Epub 2017 Aug 29.
With recognition of the biological importance of hydrogen sulfide (H S), we present a simple and effective fluorescent probe for H S using a Tb coordination polymer-Cu compound (DPA/Tb/G-Cu ). Dipicolinic acid (DPA) and guanosine (G) can coordinate with Tb to form a macromolecular coordination polymer (DPA/Tb/G). DPA/Tb/G specifically binds to Cu in the presence of coexisting cations, and obvious fluorescence quenching is observed. The quenched fluorescence can be exclusively recovered upon the addition of sulfide, which is measured in the mode of time-resolved fluorescence. The fluorescence intensities of the DPA/Tb/G-Cu compound enhance linearly with increasing sulfide concentrations from 1 to 30 μM. The detection limit for sulfide in aqueous solution is estimated to be 0.3 μM (at 3σ). The DPA/Tb/G-Cu compound was successfully applied to sense H S in human serum samples and exhibited a satisfactory result. It displays some desirable properties, such as fast detection procedure, high selectivity and excellent sensitivity. This method is very promising to be utilized for practical detection of H S in biological and environmental samples.
随着对硫化氢（H₂S）生物学重要性的认识，我们提出了一种使用Tb配位聚合物 - Cu化合物（DPA/Tb/G - Cu）的简单有效的H₂S荧光探针。吡啶二甲酸（DPA）和鸟苷（G）可以与Tb配位形成大分子配位聚合物（DPA/Tb/G）。在共存阳离子存在下，DPA/Tb/G特异性结合Cu，观察到明显的荧光猝灭。加入硫化物后，猝灭的荧光可以完全恢复，以时间分辨荧光模式进行测量。DPA/Tb/G - Cu化合物的荧光强度随着硫化物浓度从1 μM增加到30 μM而线性增强。水溶液中硫化物的检测限估计为0.3 μM（3σ）。DPA/Tb/G - Cu化合物已成功应用于人血清样品中H₂S的传感，并显示出令人满意的结果。它具有一些理想的特性，如检测过程快速、选择性高和灵敏度优异。该方法在生物和环境样品中H₂S的实际检测中具有很大的应用前景。
