Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt,
Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Pathobiology. 2021;88(3):211-217. doi: 10.1159/000510456. Epub 2020 Dec 30.
The chemokine receptor CXCR5 is selectively expressed on B cells; it is involved in lymphocyte homing and the development of normal lymphoid tissue. Its principle ligand is CXCL13 or B lymphocyte chemoattractant. Three polymorphisms in the CXCR5 gene, rs148351692 C/G, rs6421571 C/T, and rs78440425 G/A, have been identified.
To assess the genetic polymorphisms of CXCR5 and evaluate their possible contribution to the susceptibility and response to therapy of diffuse large B-cell lymphoma (DLBCL).
Fifty DLBCL (not otherwise specified) patients and 50 control subjects were included in this study. CXCR5 genotypes were determined by PCR-RFLP.
Our study revealed that the CXCR5 rs148351692 C/G and rs6421571 C/T gene polymorphisms are associated with an increased risk of developing DLBCL (OR 28.57 [95% CI 8.96-96.56] and 3.45 [1.67-11.83] respectively), while CXCR5 rs78440425 G/A showed no association with the risk of lymphoma. Moreover, the double and triple combined gene polymorphisms are associated with an increased risk of developing DLBCL of approximately 120-fold and 105-fold, respectively. CXCR5 gene polymorphisms had no significant impact on disease outcome or response to therapy.
CXCR5 gene polymorphisms could be considered a potential risk factor for the development of DLBCL.
趋化因子受体 CXCR5 选择性地表达于 B 细胞；它参与淋巴细胞归巢和正常淋巴组织的发育。其主要配体是 CXCL13 或 B 细胞趋化因子。CXCR5 基因中有三个多态性，rs148351692C/G、rs6421571C/T 和 rs78440425G/A。
评估 CXCR5 的遗传多态性，并评估其对弥漫性大 B 细胞淋巴瘤（DLBCL）易感性和治疗反应的可能贡献。
本研究纳入了 50 例 DLBCL（非特指型）患者和 50 名对照。通过 PCR-RFLP 确定 CXCR5 基因型。
我们的研究表明，CXCR5 rs148351692C/G 和 rs6421571C/T 基因多态性与 DLBCL 的发病风险增加相关（OR 分别为 28.57 [95%CI 8.96-96.56]和 3.45 [1.67-11.83]），而 CXCR5 rs78440425G/A 与淋巴瘤的发病风险无关。此外，双和三重联合基因多态性与 DLBCL 的发病风险增加分别约 120 倍和 105 倍相关。CXCR5 基因多态性对疾病结局或治疗反应无显著影响。
CXCR5 基因多态性可被视为 DLBCL 发生的潜在危险因素。
