College of Clinical Medicine, Ningxia Medical University, Yinchuan, 750004, China; Department of Laboratory Surgery, General Hospital of Ningxia Medical University, Yinchuan, 750004, China; Graduated School Ningxia Medical University, Yinchuan, Ningxia, China.
College of Clinical Medicine, Ningxia Medical University, Yinchuan, 750004, China; Department of Laboratory Surgery, General Hospital of Ningxia Medical University, Yinchuan, 750004, China; Graduated School Ningxia Medical University, Yinchuan, Ningxia, China.
Biomed Pharmacother. 2021 Jan;133:111028. doi: 10.1016/j.biopha.2020.111028. Epub 2020 Nov 28.
Aspirin is one of the most commonly prescribed medications. Evidence shows that it can even treat and prevent intestinal tumors. However, it has also caused a great deal of controversy due to its intestinal side effects. We therefore explored whether aspirin was beneficial or harmful to the intestines. We used aspirin continuously interfered with C57BL/6 J mice for 48 weeks, examining their intestinal tissues at 13, 26 and 48 weeks to determine the drug's effect on the intestines. In addition, we used flow cytometry (FCM) used to detect T cells and expression of T-cell immunoreceptor with immunoglobulin (Ig)- and tyrosine-based inhibitory motif (ITIM) domain (TIGIT) on their surfaces to determine aspirin's immunomodulatory effects. The results showed that long-term aspirin intervention could reverse damage to the intestines, an effect related to the drug's significant inhibitory effect on TIGIT. The change in TIGIT level could regulate T-cell subsets, so that counts of Cluster of Differentiation 4 (CD4)/chemokine (C-X3-C motif) receptor 3 (CXCR3) T-helper 1 (T1) cells and CD4/interleukin-4 (IL-4) T2 cells increased, while those of CD4/C-C chemokine receptor type 6 (CCR6) T17 cells and CD4/CD25 regulatory T cells (Tregs) decreased. In summary, we demonstrated that long-term aspirin intervention could inhibit TIGIT, regulating T cells to reverse damage to the intestines. Furthermore, aspirin is a potential therapy for diseases related to an increase in TIGIT.
阿司匹林是最常用的处方药物之一。有证据表明，它甚至可以治疗和预防肠道肿瘤。然而，由于其肠道副作用，它也引起了很大的争议。因此，我们探讨了阿司匹林对肠道是有益还是有害。我们连续用阿司匹林干预 C57BL/6 J 小鼠 48 周，在 13、26 和 48 周时检查其肠道组织，以确定药物对肠道的影响。此外，我们使用流式细胞术（FCM）检测 T 细胞，并检测其表面的 T 细胞免疫受体与免疫球蛋白（Ig）和酪氨酸基抑制基序（ITIM）域（TIGIT）的表达，以确定阿司匹林的免疫调节作用。结果表明，长期阿司匹林干预可以逆转肠道损伤，这种作用与药物对 TIGIT 的显著抑制作用有关。TIGIT 水平的变化可以调节 T 细胞亚群，使分化群 4（CD4）/趋化因子（C-X3-C 基序）受体 3（CXCR3）T1 辅助细胞和 CD4/白细胞介素 4（IL-4）T2 细胞的计数增加，而 CD4/C-C 趋化因子受体类型 6（CCR6）T17 细胞和 CD4/CD25 调节性 T 细胞（Tregs）的计数减少。总之，我们证明了长期阿司匹林干预可以抑制 TIGIT，调节 T 细胞逆转肠道损伤。此外，阿司匹林是一种治疗与 TIGIT 增加相关疾病的潜在疗法。
