Department of Bromatology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bialystok, ul. Mickiewicza 2D, 15-222 Bialystok, Poland(1).
Department of Bromatology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bialystok, ul. Mickiewicza 2D, 15-222 Bialystok, Poland(1).
Biomed Pharmacother. 2021 Jan;133:111090. doi: 10.1016/j.biopha.2020.111090. Epub 2020 Dec 4.
Cancer incidence rates are on the increase worldwide. The most common brain cancer in adults is glioblastoma. Currently available treatment modalities are limited and natural products such as mushrooms could enhance them. Apart from nutritional value, mushrooms are an excellent source of bioactive compounds and therefore could be used to treat various disorders. The aim of the study was to assess the anti-glioma potential of selected mushrooms on U87MG, LN-18 glioblastoma and SVGp12 normal human astroglial cell lines. The materials were Cantharellus cibarius, Coprinus comatus, Lycoperdon perlatum and Lactarius delicious. Aqueous, 70 % ethanol or 95 % ethanol extracts from mushrooms were used for analysis including assessment of antioxidant activity by DPPH assay, cell viability by MTT assay, DNA biosynthesis by thymidine incorporation assay, activity of metalloproteinase by gelatin zymography and cell cycle assay by flow cytometry. Mushroom extracts influenced the viability and DNA biosynthesis of cancer cells. Activity of ethanol mushroom extracts was stronger than that of aqueous extracts. Anti-glioma mechanism consisted in inhibition of cancer cell proliferation and induction of apoptosis associated with arrest of cells in subG1 or G2/M phase of cell cycle, and inhibition of metalloproteinases activity. Among investigated mushrooms, L. deliciosus and C. comatus showed the greatest anti-glioma potential.
癌症发病率在全球呈上升趋势。成年人中最常见的脑癌是胶质母细胞瘤。目前可用的治疗方法有限，而蘑菇等天然产物可以增强这些方法的效果。除了营养价值外，蘑菇还是生物活性化合物的极好来源，因此可用于治疗各种疾病。本研究的目的是评估几种蘑菇对 U87MG、LN-18 胶质母细胞瘤和 SVGp12 正常人类星形胶质细胞系的抗神经胶质瘤潜力。所用材料为鸡油菌、毛头鬼伞、漏斗网柄牛肝菌和美味牛肝菌。使用蘑菇的水提物、70%乙醇提取物或 95%乙醇提取物进行分析，包括 DPPH 测定法评估抗氧化活性、MTT 测定法评估细胞活力、胸苷掺入测定法评估 DNA 生物合成、明胶酶谱法评估金属蛋白酶活性以及流式细胞术评估细胞周期。蘑菇提取物影响癌细胞的活力和 DNA 生物合成。乙醇蘑菇提取物的活性强于水提物。抗神经胶质瘤的机制包括抑制癌细胞增殖和诱导与细胞周期的 subG1 或 G2/M 期阻滞相关的细胞凋亡，以及抑制金属蛋白酶活性。在所研究的蘑菇中，美味牛肝菌和毛头鬼伞表现出最大的抗神经胶质瘤潜力。
