Department of Biochemistry, Bingham University, Abuja-Keffi Expressway Road, P.M.B 005, Karu, Nigeria.
Graduate Institute for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taiwan.
Biomed Pharmacother. 2021 Jan;133:111086. doi: 10.1016/j.biopha.2020.111086. Epub 2020 Dec 11.
Testicular torsion is an acute urological emergency condition that occurs due to obstruction of blood flow to the testicles which may result in ischemia and loss of testicular functions. This study examined the protective effects of Proxeed Plus (PP), a dietary supplement on testicular ischemia/reperfusion (I/R) injured rats using oxidative stress markers, hormonal levels, apoptotic parameters, histological and immunohistochemistry analysis at 4 h and after 7 days of reperfusion. The protective treatment of the I/R injured rats with PP at 1000 and 5000 mg/kg body weight (bw) resulted in significant increases in the serum and tissue antioxidative defense capacities (superoxide dismutase, reduced glutathione, catalase, glutathione-s-transferase, and glutathione peroxidase), sex hormones (luteinizing hormone, follicle-stimulating hormone, and testosterone), also reduce pro-oxidative markers (malondialdehyde and hydrogen peroxide), serum iNOS and apoptotic parameters (Caspase -3 and Caspase -9) in comparison to the results detected in the I/R untreated rats. It was also observed that PP ameliorated histological changes of I/R injured rats; increased spermatogenetic activity, seminiferous tubular diameter, Leydig cell mass, and reduced expressions of testicular inducible nitric oxide synthase (iNOS). Therefore, the therapeutic use of Proxeed Plus could be considered a promising approach in averting testicular damage against I/R injury.
睾丸扭转是一种急性泌尿外科急症，由于睾丸血液流动受阻而发生，可能导致缺血和睾丸功能丧失。本研究使用氧化应激标志物、激素水平、凋亡参数、组织学和免疫组织化学分析，在缺血再灌注（I/R）后 4 小时和 7 天检查 Proxeed Plus（PP），一种膳食补充剂对睾丸 I/R 损伤大鼠的保护作用。在 1000 和 5000 mg/kg 体重（bw）时用 PP 对 I/R 损伤大鼠进行保护治疗，可显著增加血清和组织抗氧化防御能力（超氧化物歧化酶、还原型谷胱甘肽、过氧化氢酶、谷胱甘肽-S-转移酶和谷胱甘肽过氧化物酶）、性激素（黄体生成素、卵泡刺激素和睾酮），还降低了氧化应激标志物（丙二醛和过氧化氢）、血清诱导型一氧化氮合酶（iNOS）和凋亡参数（Caspase-3 和 Caspase-9），与未用 I/R 治疗的大鼠相比。还观察到 PP 改善了 I/R 损伤大鼠的组织学变化；增加了精子发生活性、曲细精管直径、Leydig 细胞质量，并降低了睾丸诱导型一氧化氮合酶（iNOS）的表达。因此，Proxeed Plus 的治疗用途可被认为是一种有希望的方法，可以避免 I/R 损伤引起的睾丸损伤。
