González-Martín María Inmaculada, Escuredo Olga, Hernández-Jiménez Miriam, Revilla Isabel, Vivar-Quintana Ana Ma, Martínez-Martín Iván, Hernández-Ramos Pedro
Analytical Chemistry, Nutrition and Bromatology, University of Salamanca Calle Plaza de los Caidos s/n, Salamanca, 37008, Spain.
Plant Biology and Soil Sciences. Faculty of Sciences, University of Vigo, As Lagoas, Ourense, 32004, Spain.
Talanta. 2021 Mar 1;224:121817. doi: 10.1016/j.talanta.2020.121817. Epub 2020 Oct 29.
The potential of a portable Near Infrared spectrophotometer compared with that of NIR benchtop equipment is assessed to determine theC/C relationship of stable isotopes and the fatty acid content. 105 samples of subcutaneous fat of Iberian pigs collected at the time of their slaughter have been analyzed. The analysis of stable isotopes and gas chromatography were the methods of reference used. The samples were analyzed without prior handling (portable and benchtop NIR) and after extracting the fat (benchtop NIR). The results show that with the portable equipment it is possible to determine δC (‰), 12 fatty acids, and 5 summations of fatty acids (SFA, MUFA, PUFA, w3, and w6), while with the benchtop NIR equipment it is possible to measure δC (‰), 16 fatty acids, and the 5 summationsof fatty acids. The correlation coefficients of the portable equipment were slightly lower than those of the NIR benchtop equipment.
对便携式近红外分光光度计与近红外台式设备的潜力进行了评估,以确定稳定同位素的C/C关系和脂肪酸含量。分析了105份伊比利亚猪屠宰时采集的皮下脂肪样本。稳定同位素分析和气相色谱法是所使用的参考方法。对样本进行了未经预处理(便携式和台式近红外)以及脂肪提取后(台式近红外)的分析。结果表明,使用便携式设备可以测定δC(‰)、12种脂肪酸以及5种脂肪酸总和(饱和脂肪酸、单不饱和脂肪酸、多不饱和脂肪酸、w3和w6),而使用台式近红外设备可以测量δC(‰)、16种脂肪酸以及5种脂肪酸总和。便携式设备的相关系数略低于近红外台式设备。