在来自秘鲁和墨西哥的原始番茄中发现的赋予对AAL毒素易感性的基因突变。

Mutations Found in the Gene That Confer Susceptibility to the AAL-Toxin in Ancestral Tomatoes from Peru and Mexico.

作者信息

Tsuzuki Rin, Cabrera Pintado Rosa María, Biondi Thorndike Jorge Andrés, Gutiérrez Reynoso Dina Lida, Amasifuen Guerra Carlos Alberto, Guerrero Abad Juan Carlos, Aragón Caballero Liliana Maria, Huarhua Zaquinaula Medali Heidi, Ureta Sierra Cledy, Alberca Cruz Olenka Ines, Elespuru Suna Milca Gianira, Blas Sevillano Raúl Humberto, Torres Arias Ines Carolina, Flores Ticona Joel, de Baldárrago Fátima Cáceres, Pérez Enrique Rodoríguez, Hozum Takuo, Saito Hiroki, Kotera Shunsuke, Akagi Yasunori, Kodama Motoichiro, Komatsu Ken, Arie Tsutomu

机构信息

Bio-Applications and Systems Engineering-BASE, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, Japan.

National Institute of Agricultural Innovation (INIA), La Monila 15026, Lima 12, Peru.

出版信息

Plants (Basel). 2020 Dec 28;10(1):47. doi: 10.3390/plants10010047.

DOI:10.3390/plants10010047

PMID:33379271

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7824085/

Abstract

Tomato susceptibility/resistance to stem canker disease caused by f. sp. and its pathogenic factor AAL-toxin is determined by the presence of the gene. Several cultivars of commercial tomato ( var. , ) are reported to have a mutation in , resulting in their susceptibility to AAL-toxin. We evaluated 119 ancestral tomato accessions including (), var. () and var. "jitomate criollo" () for AAL-toxin susceptibility. Three accessions, PER018805, PER018894, and M5-3, were susceptible to AAL-toxin. PER018894 and M5-3 had a two-nucleotide deletion (nt 854_855del) in identical to that found in cv. Aichi-first. Another mutation (nt 931_932insT) that may confer AAL-toxin susceptibility was identified in PER018805. In the phylogenetic tree based on the 18 COSII sequences, a clade (S3) is composed of , including the AAL-toxin susceptible PER018805, and . AAL-toxin susceptible PER018894 and M5-3 were in Clade S2 with cultivars. As is thought to be the ancestor of , and is an intermediate tomato between and , s with/without the mutation seem to have been inherited throughout the history of tomato domestication and breeding.

摘要

番茄对由f. sp.引起的茎溃疡病的易感性/抗性及其致病因子AAL-毒素由基因的存在决定。据报道，几个商业番茄品种（var., ）在中存在突变，导致它们对AAL-毒素敏感。我们评估了119份番茄原始种质，包括（）、var. （）和var. “jitomate criollo”（）对AAL-毒素的敏感性。三个种质，PER018805、PER018894和M5-3对AAL-毒素敏感。PER018894和M5-3在中存在与爱知一号品种相同的两核苷酸缺失（nt 854_855del）。在PER018805中鉴定出另一个可能导致对AAL-毒素敏感的突变（nt 931_932insT）。在基于18个COSII序列的系统发育树中，一个分支（S3）由组成，包括对AAL-毒素敏感的PER018805和。对AAL-毒素敏感的PER018894和M5-3与品种在分支S2中。由于被认为是的祖先，并且是介于和之间的中间番茄，有/无该突变的似乎在番茄驯化和育种的整个历史中都有遗传。