van der Biezen E A, Glagotskaya T, Overduin B, Nijkamp H J, Hille J
Department of Genetics, Vrije Universiteit, Amsterdam, The Netherlands.
Mol Gen Genet. 1995 May 20;247(4):453-61. doi: 10.1007/BF00293147.
The fungal pathogen Alternaria alternata f. sp. lycopersici produces AAL-toxins that function as chemical determinants of the Alternaria stem canker disease in the tomato (Lycopersicon esculentum). In resistant cultivars, the disease is controlled by the Asc locus on chromosome 3. Our aim was to characterize novel sources of resistance to the fungus and of insensitivity to the host-selective AAL-toxins. To that end, the degree of sensitivity of wild tomato species to AAL-toxins was analyzed. Of all members of the genus Lycopersicon, only L. cheesmanii was revealed to be sensitive to AAL-toxins and susceptible to fungal infection. Besides moderately insensitive responses from some species, L. pennellii and L. peruvianum were shown to be highly insensitive to AAL-toxins as well as resistant to the pathogen. Genetic analyses showed that high insensitivity to AAL-toxins from L. pennellii is inherited in tomato as a single complete dominant locus. This is in contrast to the incomplete dominance of insensitivity to AAL-toxins of L. esculentum. Subsequent classical genetics, RFLP mapping and allelic testing indicated that high insensitivity to AAL-toxins from L. pennellii is conferred by a new allele of the Asc locus.
真菌病原体链格孢菌番茄专化型（Alternaria alternata f. sp. lycopersici）产生的AAL毒素是番茄（Lycopersicon esculentum）链格孢菌溃疡病的化学致病决定因素。在抗性品种中，该病害由3号染色体上的Asc位点控制。我们的目标是鉴定对该真菌具有抗性以及对宿主选择性AAL毒素不敏感的新来源。为此，分析了野生番茄物种对AAL毒素的敏感程度。在番茄属的所有成员中，只有智利番茄（L. cheesmanii）被发现对AAL毒素敏感且易受真菌感染。除了一些物种表现出中度不敏感反应外，潘那利番茄（L. pennellii）和秘鲁番茄（L. peruvianum）对AAL毒素高度不敏感，并且对该病原体具有抗性。遗传分析表明，潘那利番茄对AAL毒素的高度不敏感性在番茄中作为一个单一的完全显性位点遗传。这与栽培番茄对AAL毒素不敏感性的不完全显性形成对比。随后的经典遗传学、RFLP图谱分析和等位基因测试表明，潘那利番茄对AAL毒素的高度不敏感性由Asc位点的一个新等位基因赋予。
