Choi Jiyoung, Moon Mi Yeon, Han Gi Yeon, Chang Moon Sik, Yang Dongki, Cha Joonseok
Research Center, The Garden of Naturalsolution, Gyeonggi-do 18103, Korea.
Department of Physiology, College of Medicine, Gachon University, Incheon 21999, Korea.
Biomolecules. 2020 Dec 28;11(1):23. doi: 10.3390/biom11010023.
Dietary supplement and personal care products aiming to provide protection from air pollution have been of great interest for decades. Epidemiology demonstrated that PM10 and PM2.5 particulate matter (PM) are an actual threat to public health worldwide, but the detailed processes of how these particles attack the cells are not fully understood. Here, we report that the measurement of intracellular calcium concentration ([Ca]) using human respiratory or skin cells can illustrate pollutant challenges by triggering Ca influx in these cells. This signal was generated by proteinase-activated receptor-2 (PAR-2), confirmed by competition analyses, and bark extract (PAE), a traditional medicine, was able to control the response and expression of PAR-2. Increase in proinflammatory cytokines and decrease in cell adhesion components could suggest a severe damage status by air pollutants and protection by PAE. Finally, we identified 4-O-feruloylquinic acid (FQA), an active compound of PAE, showing the same effects on Ca influx and PAR-2 regulation. The results presented here should help understand the underlying mechanism of PM insults and the beneficial effect of standardized PAE as dietary supplement or cosmetical ingredient.
数十年来，旨在提供空气污染防护的膳食补充剂和个人护理产品一直备受关注。流行病学表明，PM10和PM2.5颗粒物（PM）对全球公众健康构成实际威胁，但这些颗粒如何攻击细胞的详细过程尚未完全了解。在此，我们报告，使用人类呼吸道或皮肤细胞测量细胞内钙浓度（[Ca]）可以通过触发这些细胞中的钙内流来说明污染物的挑战。该信号由蛋白酶激活受体-2（PAR-2）产生，通过竞争分析得到证实，而一种传统药物黄柏提取物（PAE）能够控制PAR-2的反应和表达。促炎细胞因子的增加和细胞粘附成分的减少可能表明空气污染物造成了严重的损伤状态，而PAE具有保护作用。最后，我们鉴定出PAE的活性化合物4-O-阿魏酰奎尼酸（FQA），它对钙内流和PAR-2调节具有相同的作用。本文给出的结果应有助于理解PM损伤的潜在机制以及标准化PAE作为膳食补充剂或化妆品成分的有益作用。
