Sahoo Jashobanta, Jaiswar Santlal, Chatterjee Pabitra B, Subramanian Palani S, Jena Himanshu Sekhar
Inorganic Materials and Catalysis Division, Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Bhavnagar, Gujarat 364 002, India.
Academy of Scientific and Innovative Research (AcSIR), CSIR-CSMCRI, Bhavnagar, Gujarat 364 002, India.
Nanomaterials (Basel). 2020 Dec 28;11(1):53. doi: 10.3390/nano11010053.
The development of synthetic lanthanide luminescent probes for selective sensing or binding anions in aqueous medium requires an understanding of how these anions interact with synthetic lanthanide probes. Synthetic lanthanide probes designed to differentiate anions in aqueous medium could underpin exciting new sensing tools for biomedical research and drug discovery. In this direction, we present three mononuclear lanthanide-based complexes, EuLCl (), SmLCl (), and TbLCl (), incorporating a hexadentate aminomethylpiperidine-based nitrogen-rich heterocyclic ligand for sensing anion and establishing mechanistic insight on their binding activities in aqueous medium. All these complexes are meticulously studied for their preferential selectivities towards different anions such as HPO, SO, CHCOO, I, Br, Cl, F, NO, CO/HCO, and HSO at pH 7.4 in aqueous HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid) buffer. Among the anions scanned, HPO showed an excellent luminescence change with all three complexes. Job's plot and ESI-MS support the 1:2 association between the receptors and HPO. Systematic spectrophotometric titrations of - against HPO demonstrates that the emission intensities of and were enhanced slightly upon the addition of HPO in the range 0.01-1 equiv and 0.01-2 equiv., respectively. Among the three complexes, complex showed a steady quenching of luminescence throughout the titration of hydrogen phosphate. The lower and higher detection limits of HPO by complexes and were determined as 0.1-4 mM and 0.4-3.2 mM, respectively, while complex covered 0.2-100 μM. This concludes that all complexes demonstrated a high degree of sensitivity and selectivity towards HPO.
开发用于在水性介质中选择性传感或结合阴离子的合成镧系元素发光探针，需要了解这些阴离子如何与合成镧系元素探针相互作用。设计用于区分水性介质中阴离子的合成镧系元素探针，可为生物医学研究和药物发现提供令人兴奋的新型传感工具。在此方向上，我们展示了三种基于单核镧系元素的配合物，EuLCl（）、SmLCl（）和TbLCl（），它们包含一种基于六齿氨基甲基哌啶的富氮杂环配体，用于传感阴离子并深入了解其在水性介质中的结合活性机制。对所有这些配合物在pH 7.4的水性HEPES（2-[4-(2-羟乙基)哌嗪-1-基]乙烷磺酸）缓冲液中对不同阴离子（如HPO、SO、CHCOO、I、Br、Cl、F、NO、CO/HCO和HSO）的优先选择性进行了细致研究。在所扫描的阴离子中，HPO与所有三种配合物都表现出优异的发光变化。Job曲线和电喷雾电离质谱（ESI-MS）支持受体与HPO之间的1:2缔合。-对HPO的系统分光光度滴定表明，在分别加入0.01 - 1当量和0.01 - 2当量的HPO范围内，和的发射强度略有增强。在这三种配合物中，配合物在整个磷酸氢盐滴定过程中表现出稳定的发光猝灭。配合物和对HPO的较低和较高检测限分别确定为(0.1 - 4 mM)和(0.4 - 3.2 mM)，而配合物覆盖(0.2 - 100 μM)。这表明所有配合物对HPO都表现出高度的灵敏度和选择性。
