Liu X Z, Peng Y Z, Shen Z A
Department of Burns and Plastic Surgery, the Fourth Medical Center of PLA General Hospital, Beijing 100048, China.
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, the First Affiliated Hospital of Army Medical University (the Third Military Medical University), Chongqing 400038, China.
Zhonghua Shao Shang Za Zhi. 2020 Dec 20;36(12):1208-1212. doi: 10.3760/cma.j.cn501120-20191127-00445.
After the first single-cell RNA sequencing of human islet in 2016, a large number of analysis of RNA sequencing at single-cell level on mouse and human islets emerged, which has brought new progress to the research of islet cell biology. It has been proved that single-cell RNA sequencing can characterize rare types of endocrine cells, find the cellular heterogeneity of typical endocrine cells and new cell subtypes, analyze the differences of endocrine cells among species, and describe different states of various types of islet cells in developmental processes and metabolic diseases more accurately. Although the detection efficiency of single-cell RNA sequencing for low abundance transcripts is relatively low at present, with the improvement of technology and the progress of analytical methods, this technology will undoubtedly become a powerful tool to explore the cellular heterogeneity, development, and biological characteristics of metabolic diseases.
2016年人类胰岛首次进行单细胞RNA测序后，大量关于小鼠和人类胰岛单细胞水平的RNA测序分析不断涌现，这为胰岛细胞生物学研究带来了新进展。事实证明，单细胞RNA测序能够鉴定罕见类型的内分泌细胞，发现典型内分泌细胞的细胞异质性和新的细胞亚型，分析物种间内分泌细胞的差异，更准确地描述各类胰岛细胞在发育过程和代谢疾病中的不同状态。尽管目前单细胞RNA测序对低丰度转录本的检测效率相对较低，但随着技术的改进和分析方法的进步，这项技术无疑将成为探索代谢疾病细胞异质性、发育及生物学特性的有力工具。
