Lindholm T C, Lindholm T S, Alitalo I, Urist M R
Bone Research Group, Orthopaedic Hospital of the Invalid Foundation, Helsinki, Finland.
Clin Orthop Relat Res. 1988 Feb;227:265-8.
An aggregate of partially purified bovine bone morphogenetic protein (bBMP) and bone matrix insoluble noncollagenous proteins (iNCP), weighing a total of 100 mg of lyophilized BMP/iNCP, was implanted using ultra thin gelatin capsules in skull trephine defects in adult sheep. One hundred milligram samples of freeze-dried bovine serum albumin (BSA) were similarly implanted for controls. In five sheep, the capsules were implanted in 18-20 mm trephine skull defects and also in posterior cervical muscle pouches for heterotopic controls. In two out of five sheep, the trephines were repaired with bone as early as four weeks after the operation. Eight to 12 weeks after surgery repair was complete in the other three sheep. In the control contralateral trephines, one-third to one-half of the defect was incompletely repaired. Neither the BMP nor the BSA control implants induced bone formation in the muscle. While the BMP/iNCP prepared from bovine bone consistently induced regeneration in skull trephine defects, only fibrous tissue and no extraskeletal bone was induced to form in cervical muscle pouches in sheep.
将总量为100毫克冻干的骨形态发生蛋白/不溶性非胶原蛋白（BMP/iNCP）（其中部分纯化的牛骨形态发生蛋白（bBMP）与骨基质不溶性非胶原蛋白（iNCP）聚合而成），使用超薄明胶胶囊植入成年绵羊颅骨环锯缺损处。同样植入100毫克冻干牛血清白蛋白（BSA）样本作为对照。在五只绵羊中，将胶囊植入18 - 20毫米的颅骨环锯缺损处，同时也植入颈后肌肉袋用于异位对照。在五只绵羊中的两只中，术后仅四周骨环锯缺损处就被骨修复。另外三只绵羊在术后8至12周完成修复。在对照侧的对侧骨环锯缺损处，三分之一至二分之一的缺损未完全修复。BMP和BSA对照植入物均未在肌肉中诱导骨形成。虽然从牛骨制备的BMP/iNCP始终能诱导颅骨环锯缺损处再生，但在绵羊的颈后肌肉袋中仅诱导形成纤维组织，未诱导形成骨骼外骨。
