狐源犬腺病毒 1 型的生物学特性及感染 MDCK 细胞的转录组分析。
The biological characteristics of the canine adenovirus type 1 from fox and the transcriptome analysis of the infected MDCK cell.
机构信息
Key Laboratory of Special Animal Epidemic Disease, Ministry of Agriculture, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
Pharmaron Beijing Co., Ltd., Beijing, China.
出版信息
Cell Biol Int. 2021 May;45(5):936-947. doi: 10.1002/cbin.11537. Epub 2021 Jan 21.
Canine adenovirus type 1 (CAdV-1) is the etiologic agent of fox encephalitis, and a virus strain from fox encephalitis is isolated and related research are conducted. In this experiment, the results showed that the F1301 strain was confirmed to be the CAdV-1. The whole genome of the CAdV-1 F1301 strain isolated from fox was 30,535 bp and had higher homology to the other reported CAdV-1 strains. After 0, 12, and 36 h of CAdV-1 infection, the difference gene of the 592 long noncoding RNA and 11,215 microRNA were involved in cell responses to CAdV-1 infection through the PI3K-AKT, Wnt, Herpes simplex, hepatitis C, and Epstein-Barr virus infection pathway in Madin-Darby canine kidney cell line (MDCK). The results indicate that the biological characterization of the CAdV-1 and the MDCK cell-CAdV-1 interaction are clarified.
犬腺病毒 1 型（CAdV-1）是狐狸脑炎的病原体，分离出一种来自狐狸脑炎的病毒株并进行了相关研究。在本实验中，结果表明 F1301 株被确认为 CAdV-1。从狐狸中分离出的 CAdV-1 F1301 株的全基因组为 30535bp，与其他报道的 CAdV-1 株具有更高的同源性。在 CAdV-1 感染后 0、12 和 36 小时，通过 Madin-Darby 犬肾细胞系（MDCK）中的 PI3K-AKT、Wnt、单纯疱疹、丙型肝炎和 Epstein-Barr 病毒感染途径，涉及 592 个长非编码 RNA 和 11215 个 microRNA 的差异基因参与细胞对 CAdV-1 感染的反应。结果表明，阐明了 CAdV-1 的生物学特征和 MDCK 细胞-CAdV-1 相互作用。
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