Arecanut ( L.) Seed Polyphenol-Ameliorated Osteoporosis by Altering Gut Microbiome via LYZ and the Immune System in Estrogen-Deficient Rats.

Polyphenol can improve osteoporosis and is closely associated with gut microbiota, while the mechanism and the relationship among polyphenol, osteoporosis, and gut microbiota colonization remain unclear. Here, an osteoporosis rat model established by ovariectomy was employed to investigate the improving mechanism of arecanut ( L.) seed polyphenol (ACP) on osteoporosis by regulating gut microbiota. We analyzed the bone microstructure, Paneth cells, regulating microbial protein (lysozyme (LYZ)), proinflammatory cytokines, macrophage infiltration levels, and gut microbial communities in a rat. ACP improved the trabecular microstructure compared to OVX, including the increased trabecular number (Tb.N) ( < 0.01) and trabecular thickness (Tb.Th) ( < 0.001) and decreased trabecular separation (Tb.Sp) ( < 0.01). At the phylum level, Bacteroidetes was increased after ovariectomy ( < 0.001) and Firmicutes and Proteobacteria were increased in ACP ( < 0.001). Antiosteoporosis groups with lower LYZ and Paneth cells ( < 0.001) showed that the microbiota , which have a negative effect on bone metabolism were decreased in ACP ( 0.001). Altogether, these studies showed that the estrogen deficiency could induce the shedding of Paneth cells, which leads to the decrease of LYZ, while ACP could increase the LYZ expression by maintaining the population of Paneth cells in an estrogen-deficient host, which were implicated in gut microbiota regulation and improved osteoporosis by controlling the inflammatory reaction.