Harrison R A, Farries T C, Northrop F D, Lachmann P J, Davis A E
MITI Unit, MRC Centre, Cambridge, UK.
Complement. 1988;5(1):27-32. doi: 10.1159/000463028.
C3f, a peptide presumed to be generated by the combined actions of factors I and H on fluid-phase C3b, has been isolated and sequenced. The peptide is 17 residues long and has a molecular weight of 1,847 daltons. The amino-terminal sequence is, with the exception of a single residue, identical to that deduced for the 46-kilodalton polypeptide seen transiently in the generation of iC3b from C3b, and is in full agreement with the sequence deduced from cDNA analysis. In addition, high-pressure liquid chromatography of the digestion of C3b by factor I has shown that C3f is the sole peptide released during iC3b generation.
C3f是一种据推测由I因子和H因子对液相C3b共同作用产生的肽段，现已被分离并测序。该肽段长度为17个残基，分子量为1847道尔顿。其氨基末端序列，除了一个残基外，与从C3b生成iC3b过程中短暂出现的46千道尔顿多肽推导的序列相同，并且与cDNA分析推导的序列完全一致。此外，I因子对C3b消化产物的高压液相色谱分析表明，C3f是生成iC3b过程中释放的唯一肽段。
