German Center for Neurodegenerative Diseases (DZNE e.V.), 89081 Ulm, Germany.
Department of Neurology, Ulm University Hospital, 89081 Ulm, Germany.
Int J Mol Sci. 2022 Jun 30;23(13):7332. doi: 10.3390/ijms23137332.
Mass spectrometry (MS), with its immense technological developments over the last two decades, has emerged as an unavoidable technique in analyzing biomolecules such as proteins and peptides. Its multiplexing capability and explorative approach make it a valuable tool for analyzing complex clinical samples concerning biomarker research and investigating pathophysiological mechanisms. Peptides regulate various biological processes, and several of them play a critical role in many disease-related pathological conditions. One important example in neurodegenerative diseases is the accumulation of amyloid-beta peptides (Aβ) in the brain of Alzheimer's disease (AD) patients. When investigating brain function and brain-related pathologies, such as neurodegenerative diseases, cerebrospinal fluid (CSF) represents the most suitable sample because of its direct contact with the brain. In this review, we evaluate publications applying peptidomics analysis to CSF samples, focusing on neurodegenerative diseases. We describe the methodology of peptidomics analysis and give an overview of the achievements of CSF peptidomics over the years. Finally, publications reporting peptides regulated in AD are discussed.
质谱（MS）在过去二十年中经历了巨大的技术发展，已成为分析蛋白质和肽等生物分子的不可或缺的技术。其多重分析能力和探索性方法使其成为分析涉及生物标志物研究和调查病理生理机制的复杂临床样本的有价值的工具。肽调节着各种生物过程，其中一些肽在许多与疾病相关的病理条件中起着关键作用。在神经退行性疾病中，一个重要的例子是淀粉样β肽（Aβ）在阿尔茨海默病（AD）患者大脑中的积累。当研究大脑功能和与大脑相关的病理学，如神经退行性疾病时，脑脊液（CSF）是最适合的样本，因为它与大脑直接接触。在这篇综述中，我们评估了应用于 CSF 样本的肽组学分析的出版物，重点关注神经退行性疾病。我们描述了肽组学分析的方法，并概述了多年来 CSF 肽组学的成就。最后，讨论了报告 AD 中调节肽的出版物。
