Li Tuo, Jin Wei, He Yi, Zhou Zhongjun
Department of Endocrinology, Chang Zheng Hospital, Shanghai 200003, China; School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
Stem Cell Res. 2021 Jan;50:102136. doi: 10.1016/j.scr.2020.102136. Epub 2020 Dec 28.
PRMT6 is a type I protein arginine methyltransferase (PRMT) which participates in diverse biological processes. To facilitate the understanding of PRMT6 functions, we generated two independent homozygous PRMT6 knockout Human ESCs clones, PRMT6-KO-10 and PRMT6-KO-24, in human WA01 ES cells by CRISPR/Cas9. The pluripotency of both clones was verified by the presence of pluripotent markers, normal karyotypes, and differentiation potential in vivo whereas the lack of PRMT6 expression was demonstrated by sequencing and detection of the significant changes in specific histone methylation patterns.
PRMT6是一种I型蛋白质精氨酸甲基转移酶（PRMT），参与多种生物学过程。为了便于理解PRMT6的功能，我们通过CRISPR/Cas9在人WA01胚胎干细胞中生成了两个独立的纯合PRMT6敲除人胚胎干细胞克隆，即PRMT6-KO-10和PRMT6-KO-24。通过多能性标志物的存在、正常核型以及体内分化潜能验证了这两个克隆的多能性，而通过测序和检测特定组蛋白甲基化模式的显著变化证明了PRMT6表达的缺失。
