Department of Chemical and Materials Engineering, Chang Gung University, 259 Wen-Hwa First Road, Kwei-Shan, Tao-Yuan, 333, Taiwan.
Department of Chemical and Materials Engineering, Chang Gung University, 259 Wen-Hwa First Road, Kwei-Shan, Tao-Yuan, 333, Taiwan; Research Center for Chinese Herbal Medicine and Research Center for Food and Cosmetic Safety, College of Human Ecology, Chang Gung University of Science and Technology, 261 Wen-Hwa First Road, Taoyuan, Taiwan; Department of Chemical Engineering, Ming Chi University of Technology, 84 Gung-Juan Road, New Taipei City, Taiwan; Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou, 5 Fu-Hsing Street, Taoyuan, Taiwan.
Anal Chim Acta. 2021 Jan 25;1143:84-92. doi: 10.1016/j.aca.2020.11.045. Epub 2020 Nov 30.
An electrochemical platform based on a screen-printed carbon electrode (SPCE) is developed to detect parathyroid hormone (PTH). A nanocomposite of multi-walled carbon nanotube (MWCNT) and gold nanoparticles (AuNP) was deposited on the SPCE to immobilize antibodies and horseradish peroxidase (HRP). MWCNT improved the stability and conductivity of the immunosensor because of its good electron-transfer ability and tubular structure. The AuNP not only provided a large surface area for antibody immobilization, but it also enhanced the electrochemical signal for enzyme-linked immunosensing. Cyclic voltammetry showed both electron transfer and the effective surface area were increased on the modified electrode. The characteristics of the modified SPCE were assayed by Raman spectroscopy, scanning electron microscopy, atomic force microscopy, and electrochemical techniques. The linear detection range of this PTH immunosensor was within 1-300 pg/ml, and the electrochemical performance was not affected by interference from protein components in human serum. After storage at 4 °C for 28 days, 85% PTH sensing ability of this immunosensor was maintained compared to the freshly prepared one using the SWV and DPV methods. The relative standard deviations of all measurements were within 3-8% for both voltammetric methods. These results indicated the developed immunosensor had good stability and reproducibility. This PTH immunosensor had a detection limit of 0.886 and 0.065 pg/ml for the differential pulse voltammetry and square wave voltammetry, respectively. We provided a quick analysis of serum PTH which might be used as an electrochemical immunosensing platform for point-of-care testing.
一种基于丝网印刷碳电极（SPCE）的电化学平台被开发出来用于检测甲状旁腺激素（PTH）。多壁碳纳米管（MWCNT）和金纳米粒子（AuNP）的纳米复合材料被沉积在 SPCE 上以固定抗体和辣根过氧化物酶（HRP）。MWCNT 由于其良好的电子传递能力和管状结构，提高了免疫传感器的稳定性和导电性。AuNP 不仅为抗体固定提供了较大的表面积，而且还增强了酶联免疫传感的电化学信号。循环伏安法表明，在修饰电极上增加了电子转移和有效表面积。通过拉曼光谱、扫描电子显微镜、原子力显微镜和电化学技术对修饰后的 SPCE 的特性进行了检测。该 PTH 免疫传感器的线性检测范围为 1-300 pg/ml 以内，电化学性能不受人血清中蛋白质成分的干扰。在 4°C 下储存 28 天后，与使用 SWV 和 DPV 方法制备的新鲜免疫传感器相比，该免疫传感器对 PTH 的传感能力保持了 85%。两种伏安法的所有测量的相对标准偏差均在 3-8%范围内。这些结果表明，所开发的免疫传感器具有良好的稳定性和重现性。该 PTH 免疫传感器对差分脉冲伏安法和方波伏安法的检测限分别为 0.886 和 0.065 pg/ml。我们提供了一种快速分析血清 PTH 的方法，该方法可能用作即时检测的电化学免疫传感平台。
