Major in Materials Science and Engineering, Hallym University, Chuncheon 24252, Gangwon-do, Korea.
Cooperative Course of Nano-Medical Device Engineering, Hallym University, Chuncheon 24252, Gangwon-do, Korea.
Biosensors (Basel). 2021 Sep 29;11(10):360. doi: 10.3390/bios11100360.
Biosensors used for medical diagnosis work by analyzing physiological fluids. Antibodies have been frequently used as molecular recognition molecules for the specific binding of target analytes from complex biological solutions. Electrochemistry has been introduced for the measurement of quantitative signals from transducer-bound analytes for many reasons, including good sensitivity. Recently, numerous electrochemical immunosensors have been developed and various strategies have been proposed to detect biomarkers. In this paper, the recent progress in electrochemical immunosensors is reviewed. In particular, we focused on the immobilization methods using antibodies for voltammetric, amperometric, impedimetric, and electrochemiluminescent immunosensors.
用于医学诊断的生物传感器通过分析生理流体来工作。抗体经常被用作分子识别分子，用于从复杂的生物溶液中特异性结合目标分析物。电化学由于其具有良好的灵敏度等诸多原因，已经被引入到用于测量转换器结合的分析物的定量信号中。最近，已经开发了许多电化学免疫传感器，并提出了各种策略来检测生物标志物。本文综述了电化学免疫传感器的最新进展。特别是，我们专注于使用抗体的固定化方法，用于伏安法、安培法、阻抗法和电化学发光免疫传感器。
