Usak University Dentistry Faculty Department of Oral and Maxillofacial Surgery, Uşak, Turkey.
Usak Training and Research Hospital, Department of Physical Therapy and Rehabilitation, Usak, Turkey.
J Craniomaxillofac Surg. 2021 Feb;49(2):98-103. doi: 10.1016/j.jcms.2020.12.004. Epub 2020 Dec 17.
The aim of the present study was to evaluate the effects of a single dose of locally administered teriparatide (TP) on healing critical-sized defects in rat mandibles through histomorphometric and microcomputed tomography (micro-CT) analyses. In this study, 48 Sprague-Dawley rats were used. The experimental animals were divided into 4 groups as follows: Group 1 had empty defects, Group 2 received autografts, Group 3 received allografts, and Group 4 received allografts combined with 40 μg of TP. Eight weeks after the surgical procedure, all rats were sacrificed, and all specimens were evaluated using micro-CT and histomorphometric analyses. The results of the histomorphometric analysis showed that Group 4 had the most new bone area (0.85 mm ± 0.13 mm) (p = 0.002) and the highest number of osteoblasts (86.61 ± 4.86) (p = 0.001). In addition, the results of the micro-CT analysis showed that Group 4 had the highest bone volume/total volume (23.27% ± 0.15%) (p = 0.001). The histomorphometric and micro-CT values of Group 2 were higher than those of Group 1 but lower than those of Group 3 and Group 4. The results of the study show that a single dose of locally administered TP has a positive effect on the integration of allografts. However, further studies are necessary to identify the mechanism of action and the effective minimum and maximum doses of locally administered TP.
本研究旨在通过组织形态计量学和微计算机断层扫描（micro-CT）分析评估单次局部给予特立帕肽（TP）对大鼠下颌骨临界尺寸缺损愈合的影响。本研究使用了 48 只 Sprague-Dawley 大鼠。实验动物分为 4 组：第 1 组为空白缺损组，第 2 组接受自体移植物，第 3 组接受同种异体移植物，第 4 组接受同种异体移植物联合 40μg 的 TP。手术 8 周后，所有大鼠均被处死，所有标本均通过 micro-CT 和组织形态计量学分析进行评估。组织形态计量学分析的结果显示，第 4 组的新骨面积（0.85mm±0.13mm）最大（p=0.002），成骨细胞数量最多（86.61±4.86）（p=0.001）。此外，micro-CT 分析的结果表明，第 4 组的骨体积/总体积最高（23.27%±0.15%）（p=0.001）。第 2 组的组织形态计量学和 micro-CT 值高于第 1 组，但低于第 3 组和第 4 组。研究结果表明，单次局部给予 TP 对同种异体移植物的整合具有积极影响。然而，需要进一步的研究来确定局部给予 TP 的作用机制和有效最小和最大剂量。
