Proteomic Analysis of Stationary Growth Stage Adaptation and Nutritional Deficiency Response of .

Brucellosis, an important bacterial zoonosis caused by species, has drawn increasing attention worldwide. As an intracellular pathogen, the ability of to deal with stress within the host cell is closely related to its virulence. Due to the similarity between the survival pressure on within host cells and that during the stationary phase, a label-free proteomics approach was used to study the adaptive response of in the stationary stage to reveal the possible intracellular adaptation mechanism in this study. A total of 182 downregulated and 140 upregulated proteins were found in the stationary-phase . adapted to adverse environmental changes by regulating virulence, reproduction, transcription, translation, stress response, and energy production. In addition, both exponential- and stationary-phase were treated with short-term starvation. The exponential restricted cell reproduction and energy utilization and enhanced material transport in response to nutritional stress. Compared with the exponential phase, stationary adjusted their protein expression to a lesser extent under starvation. Therefore, in the two growth stages significantly differed in the regulation of protein expression in response to the same stress. Overall, we outlined the adaptive mechanisms that may employ during growth and compared the differences between exponential- and stationary-phase in response to starvation.