Department of Systems Aging Science and Medicine, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan; Department of Comprehensive Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan; Department of Pathology, Toranomon Hospital, Tokyo, Japan.
Department of Systems Aging Science and Medicine, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
Clin Breast Cancer. 2021 Oct;21(5):399-407.e2. doi: 10.1016/j.clbc.2020.12.001. Epub 2020 Dec 9.
We previously identified estrogen-responsive finger protein (Efp) as an estrogen-induced gene, and showed that the positive immunoreactivity of Efp is a poor prognostic factor for patients with breast cancer. We also demonstrated that Efp has distinctive roles in innate immunity by activating pattern recognition receptor retinoic acid-inducible gene I (RIG-I). The clinical value of RIG-I protein expression in breast cancer had not been evaluated in relationship with patients' prognosis.
Tissue samples of estrogen receptor-positive invasive breast cancer were obtained from 145 female patients with breast cancer who underwent surgical treatment. Immunoreactivities of RIG-I and Efp were analyzed with the antibodies generated for the present study.
Positive immunoreactivity of RIG-I was correlated with lower disease-free survival (P = .032) and was an independent poor prognostic factor (P = .043). RIG-I immunoreactivity was positively correlated with that of Efp (P = .0004). Patients with positive immunoreactivities of both RIG-I and Efp proteins were associated with a lower disease-free survival rate (P = .005).
Positive immunoreactivity of RIG-I has clinical significance as a poor prognostic factor in patients with estrogen receptor-positive breast cancer. A positive correlation of RIG-I and Efp immunoreactivities was observed, and the combination of their immunoreactivities can be used to predict patients' prognosis.
我们之前发现雌激素反应指状蛋白（Efp）是雌激素诱导的基因，并表明 Efp 的阳性免疫反应是乳腺癌患者的预后不良因素。我们还证明，Efp 通过激活模式识别受体视黄酸诱导基因 I（RIG-I）在先天免疫中具有独特的作用。尚未评估 RIG-I 蛋白在乳腺癌中的表达与患者预后的关系的临床价值。
从 145 名接受手术治疗的女性乳腺癌患者中获得雌激素受体阳性浸润性乳腺癌的组织样本。使用为本研究生成的抗体分析 RIG-I 和 Efp 的免疫反应性。
RIG-I 的阳性免疫反应与无病生存率降低相关（P =.032），并且是独立的预后不良因素（P =.043）。RIG-I 免疫反应性与 Efp 的免疫反应性呈正相关（P =.0004）。同时具有 RIG-I 和 Efp 蛋白阳性免疫反应的患者无病生存率较低（P =.005）。
RIG-I 的阳性免疫反应作为雌激素受体阳性乳腺癌患者的预后不良因素具有临床意义。观察到 RIG-I 和 Efp 免疫反应性的正相关，并且可以将它们的免疫反应性组合用于预测患者的预后。
